Fu W, Xiao G, Yu P
Institute of Burn Research, Southwestern Hospital, Third Military Medical College, Chongqing.
Zhonghua Yi Xue Za Zhi. 1995 Mar;75(3):159-60, 190-1.
For developing monoclonal antibodies (MAb) that bound to lipid A of the LPS molecule, we established several hybridomas cell lines by fusion of SP2/0 and spleen cells from BALB/c mice immunized by J5 mutant strain of E. coli 0111:B4. Each MAb exhibited a high titer by ELISA assay. Six of MAb were of immunoglobulin G isotype and another one immunoglobulin M. All of these MAb demonstrated different capacity to cross-react with several gram-negative bacteria and their endotoxins. Among them, 9G6 MAb improved the survival rate of mice significantly when administered 2 hours before the challenge by pseudomonas A and E. coli. These findings suggest that MAb will probably be useful in the treatment of sepsis caused by a wide variety of gram-negative bacteria.
为了开发与脂多糖（LPS）分子的脂质A结合的单克隆抗体（MAb），我们通过将SP2/0细胞与经大肠杆菌0111:B4的J5突变株免疫的BALB/c小鼠的脾细胞融合，建立了多个杂交瘤细胞系。通过酶联免疫吸附测定（ELISA），每个单克隆抗体都表现出高滴度。六个单克隆抗体属于免疫球蛋白G同种型，另一个属于免疫球蛋白M。所有这些单克隆抗体在与几种革兰氏阴性菌及其内毒素交叉反应方面表现出不同的能力。其中，9G6单克隆抗体在受到铜绿假单胞菌和大肠杆菌攻击前2小时给药时，能显著提高小鼠的存活率。这些发现表明，单克隆抗体可能对治疗由多种革兰氏阴性菌引起的败血症有用。
