Wieler L H, Franke S, Menge C, Rose M, Bauerfeind R, Karch H, Baljer G
Institut für Hygiene und Infektionskrankheiten der Tiere der Justus-Liebig-Universität Giessen.
Dtsch Tierarztl Wochenschr. 1995 Jan;102(1):40-3.
An outbreak of edema disease (ED) was monitored in 80 piglets after weaning over a period of 4 weeks. The shedding of Shiga-like toxin-IIe) producing Escherichia coli strains, the serum bactericidal activity (SBA) against SLTEC-IIe, and the antibody response against SLT-IIe were investigated. The antibody response was monitored by utilizing a glutathione-S-transferase (GST) + SLT-IIe B/SUB fusion protein (FRANKE et al., in press) for immunoblot assays. E. coli-strain GO15III (0141:K85ac) was diagnosed as SLT-IIe-producing E. coli by polymerase chain reaction, DNA hybridization and cytotoxicity assays. Maximum excretion of GO15III appeared between days 8 and 15 after weaning. On day 1 after weaning no piglet shed GO15III, while the number increased on day 8 to 53 (66.2%) and on day 15 to 59 (73.8%) of the piglets. 4 week after weaning, GO15III was only isolated from 23 (28.8%) of the piglets. In parallel, serum bactericidal activity against GO15III increased significantly in the sera of 73 (91.2%) piglets, reaching a stable maximum from day 15 on. During the first two weeks after weaning, no piglet yielded detectable SLT-IIe-IgG. However, the number of SLT-IIe-IgG positive piglets increased steadily from day 15. On day 15, 5 (6.2%) piglets were positive in SLT-IIe immunoblot analysis and 29 days after weaning the number increased to 31 (38.8%). These data represent the first serological monitoring of a natural outbreak of edema disease in piglets after weaning by using a recombinant fusion protein (GST+SLT-IIe B/SUB). The recombinant protein proved to be a useful diagnostical tool for monitoring the specific antibody status of piglets.
在4周时间内，对80头断奶仔猪的水肿病（ED）暴发情况进行了监测。研究了产志贺样毒素IIe（SLT-IIe）的大肠杆菌菌株的排出情况、针对产志贺样毒素大肠杆菌IIe（SLTEC-IIe）的血清杀菌活性（SBA）以及针对SLT-IIe的抗体反应。通过利用谷胱甘肽-S-转移酶（GST）+SLT-IIe B/SUB融合蛋白（FRANKE等人，即将发表）进行免疫印迹分析来监测抗体反应。通过聚合酶链反应、DNA杂交和细胞毒性试验，将大肠杆菌菌株GO15III（0141:K85ac）诊断为产SLT-IIe的大肠杆菌。GO15III的最大排出量出现在断奶后第8天至第15天之间。断奶后第1天，没有仔猪排出GO15III，而在第8天，排出的仔猪数量增加到53头（66.2%），在第15天增加到59头（73.8%）。断奶4周后，仅从23头（28.8%）仔猪中分离出GO15III。与此同时，73头（91.2%）仔猪血清中针对GO15III的血清杀菌活性显著增加，从第15天开始达到稳定的最大值。在断奶后的前两周，没有仔猪产生可检测到的SLT-IIe-IgG。然而，SLT-IIe-IgG阳性仔猪的数量从第15天开始稳步增加。在第15天，5头（6.2%）仔猪的SLT-IIe免疫印迹分析呈阳性，断奶后29天，这一数量增加到31头（38.8%）。这些数据代表了首次使用重组融合蛋白（GST+SLT-IIe B/SUB）对断奶仔猪水肿病自然暴发进行的血清学监测。该重组蛋白被证明是监测仔猪特异性抗体状态的有用诊断工具。
