Dwoskin L P, Teng L, Buxton S T, Ravard A, Deo N, Crooks P A
College of Pharmacy, University of Kentucky, Lexington 40536, USA.
Eur J Pharmacol. 1995 Mar 24;276(1-2):195-9. doi: 10.1016/0014-2999(95)00077-x.
In addition to S(-)-nicotine, several minor tobacco alkaloids ((+/-)-nornicotine, anabaseine, S(-)-anabasine, and S(-)-N-methylanabasine) are present in tobacco smoke. This study demonstrates that these alkaloids increase fractional 3H release in a concentration-dependent manner from rat striatal slices preloaded with [3H]dopamine, with desensitization of this response. The rank order of EC50 values was S(-)-nicotine (3.0 +/- 2.2 microM) > (+/-)-nornicotine (6.7 +/- 2.1 microM) > anabaseine (15.4 +/- 6.1 microM) = S(-)-N-methylanabasine (16.3 +/- 4.7 microM) = S(-)-anabasine (19.3 +/- 3.2 microM). The alkaloids did not modulate fractional 3H release evoked by electrical-field depolarization. Thus, minor tobacco alkaloids may contribute to the apparent neuroprotective effects of smoking in neurodegenerative diseases.
除了S(-)-尼古丁外，烟草烟雾中还存在几种次要的烟草生物碱（(+/-)-去甲烟碱、新烟草碱、S(-)-假木贼碱和S(-)-N-甲基假木贼碱）。本研究表明，这些生物碱以浓度依赖的方式增加预先用[3H]多巴胺加载的大鼠纹状体切片中3H的分数释放，并使这种反应脱敏。EC50值的顺序为S(-)-尼古丁（3.0 +/- 2.2 microM）>(+/-)-去甲烟碱（6.7 +/- 2.1 microM）>新烟草碱（15.4 +/- 6.1 microM）=S(-)-N-甲基假木贼碱（16.3 +/- 4.7 microM）=S(-)-假木贼碱（19.3 +/- 3.2 microM）。这些生物碱不会调节电场去极化引起的3H分数释放。因此，次要的烟草生物碱可能有助于吸烟在神经退行性疾病中明显的神经保护作用。
