Nimtz M, Wray V, Rüdiger A, Conradt H S
Department of Molecular and Instrumental Structure Research, GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
FEBS Lett. 1995 May 29;365(2-3):203-8. doi: 10.1016/0014-5793(95)00473-m.
A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensional NMR techniques revealed a high mannose type structure (Man6) with a phospho-diesterbridged N-acetylglucosamine as follows: [formula: see text]
从幼仓鼠肾（BHK-21）细胞表达的重组人促红细胞生成素的糖基化位点I（Asn-24）分离出一种抗唾液酸酶的单电荷N-聚糖，其在该糖基化位点的寡糖物质中约占2-4%。质谱以及一维和二维核磁共振技术揭示了一种具有磷酸二酯桥连的N-乙酰葡糖胺的高甘露糖型结构（Man6），如下所示：[分子式：见正文]
