Scanga S, Manoukian A, Larsen E
Department of Zoology, University of Toronto, Ontario, Canada.
Dev Biol. 1995 Jun;169(2):673-82. doi: 10.1006/dbio.1995.1178.
The response of the antenna imaginal disc to ectopic Antennapedia gene expression was explored in a heat shock Antennapedia (hsAntp) transgenic strain and in strains doubly transgenic for hsAntp and downstream enhancer trap targets. The distal to proximal changes in morphological transformation in response to Antennapedia product at different developmental stages were correlated with changing expression patterns of transgene targets from antenna to leg-like patterns. Dose-response studies indicated changing thresholds of response to Antennapedia. At particular stages and doses of Antennapedia product, cell differentiation of leg bristles was uncoupled from transformation of the third antennal segment to tarsus. The results suggest that determination for bristle type does not depend on a prior determination decision for organ type. The results also provide an avenue for exploring the nature of "competence" at cellular and molecular levels.
在热休克触角足（hsAntp）转基因品系以及hsAntp和下游增强子陷阱靶点双转基因品系中，研究了触角成虫盘对外源触角足基因表达的反应。在不同发育阶段，触角对触角足产物反应的形态转化从远端到近端的变化，与转基因靶点从触角模式到腿状模式的表达模式变化相关。剂量反应研究表明对触角足的反应阈值在变化。在触角足产物的特定阶段和剂量下，腿部刚毛的细胞分化与第三触角节向跗节的转化解偶联。结果表明，刚毛类型的决定并不依赖于器官类型的先前决定。这些结果还为在细胞和分子水平上探索“感受态”的本质提供了一条途径。
