Xavier R, Nakamura M, Kobayashi S, Ishikura H, Tanigawa Y
Third Division of Internal Medicin, Shimane Medical University, Izumo, Japan.
Immunobiology. 1995 Feb;192(3-4):262-71. doi: 10.1016/S0171-2985(11)80102-6.
The human nonspecific suppressor factor (hNSF), the probable counterpart of the murine monoclonal nonspecific suppressor factor (MNSF), has been isolated from the ascitic fluid of a patient with systemic lupus erythematosus and characterized. hNSF presents an inhibitory activity on the proliferation and IgG production of mitogen stimulated human PBMC. In the present study, we demonstrate that hNSF can be isolated from the supernatants of ConA-activated T cells, but not from CD8-depleted T cells, indicating the CD8+ T cells are the major source of the factor. We also studied the effects of hNSF on purified human B and T cells; hNSF strongly inhibited the proliferation and Ig secretion by highly purified B cells induced by SAC plus IL-2, as well as the proliferation of T cells activated by Con A plus IL-2. These results indicate that hNSF is a CD8+ T cell product with strong antigen-nonspecific immunoregulatory action on both lymphocyte populations.
人类非特异性抑制因子（hNSF）可能是小鼠单克隆非特异性抑制因子（MNSF）的对应物，已从一名系统性红斑狼疮患者的腹水中分离并进行了表征。hNSF对丝裂原刺激的人外周血单个核细胞（PBMC）的增殖和IgG产生具有抑制活性。在本研究中，我们证明hNSF可以从ConA激活的T细胞上清液中分离出来，但不能从CD8缺失的T细胞中分离出来，这表明CD8 + T细胞是该因子的主要来源。我们还研究了hNSF对纯化的人B细胞和T细胞的影响；hNSF强烈抑制由SAC加IL-2诱导的高度纯化B细胞的增殖和Ig分泌，以及由Con A加IL-2激活的T细胞的增殖。这些结果表明，hNSF是一种CD8 + T细胞产物，对两种淋巴细胞群体均具有强大的抗原非特异性免疫调节作用。
