Gräber S, Lyon M K
University of Colorado, Boulder 80309, USA.
J Microsc. 1995 May;178(Pt 2):120-4. doi: 10.1111/j.1365-2818.1995.tb03587.x.
Different fractions of a mixture of protein-pigment complexes have been separated from one another by non-denaturing gel electrophoresis. These complexes were prepared for observation by inserting electron microscope grids directly into the focused bands of the pigment-protein complexes and resuming electrophoresis for a brief time, so that the complexes were deposited onto the grids. It was found that complexes deposited from each band exhibited distinctly different appearances. It was also found that the exact conditions of electrophoretic deposition onto the grids affected the appearance of the complexes. The protein-pigment complexes were characterized additionally by spectroscopy and denaturing gel electrophoresis.
通过非变性凝胶电泳已将蛋白质 - 色素复合物混合物的不同组分彼此分离。制备这些复合物用于观察的方法是，将电子显微镜网格直接插入色素 - 蛋白质复合物的聚焦条带中，并短暂恢复电泳，以便复合物沉积在网格上。结果发现，从每个条带沉积的复合物呈现出明显不同的外观。还发现，在网格上进行电泳沉积的确切条件会影响复合物的外观。此外，通过光谱学和变性凝胶电泳对蛋白质 - 色素复合物进行了表征。
