Kawanishi K, Ishida T, Kajikawa T, Tada S
Department of Clinical Laboratory, Kagawa Medical School.
Rinsho Byori. 1995 May;43(5):454-9.
Microalbuminuria, transferrin and IgG were determined in the urine of diabetic patients with normal renal function. Microalbuminuria was assayed by the immunoturbidity method, transferrin by the latex agglutination method and IgG by the ELISA method using biotin labelled IgG.HRP labelled protein G. The correlation between microalbuminuria and urinary transferrin concentrations was high (r = 0.902). However, a low titer of transferrinuria was observed, (25.5%) among the patients with microalbuminuria; a wider distribution of transferrinuria was noted than microalbuminuria. Determination of transferrinuria did not appear to be superior to the determination of microalbuminuria in early diabetic nephropathy. The urinary IgG levels assayed by ELISA using biotin labelled IgG.HRP labelled protein G method were distributed from zero to 5 mg/g Cr in the patients with normoalbuminuria. The reference intervals of the urinary IgG concentration should be settled below 5 mg/g Cr. In the patients with microalbuminuria, the concentration of urinary IgG showed a wide variation and no correlation between concentrations of microalbuminuria and urinary IgG was noted (r = 0.02). Determination of urinary IgG is not useful as an early diagnostic procedure for diabetic nephropathy.
对肾功能正常的糖尿病患者的尿液进行微量白蛋白尿、转铁蛋白和IgG检测。微量白蛋白尿采用免疫比浊法检测，转铁蛋白采用乳胶凝集法检测，IgG采用酶联免疫吸附测定法（ELISA法）检测，该方法使用生物素标记的IgG和辣根过氧化物酶标记的蛋白G。微量白蛋白尿与尿转铁蛋白浓度之间的相关性较高（r = 0.902）。然而，在微量白蛋白尿患者中观察到低滴度的转铁蛋白尿（25.5%）；与微量白蛋白尿相比，转铁蛋白尿的分布范围更广。在早期糖尿病肾病中，转铁蛋白尿的检测似乎并不优于微量白蛋白尿的检测。采用生物素标记的IgG和辣根过氧化物酶标记的蛋白G的ELISA法检测的尿IgG水平，在尿白蛋白正常的患者中为0至5 mg/g肌酐。尿IgG浓度的参考区间应设定在5 mg/g肌酐以下。在微量白蛋白尿患者中，尿IgG浓度变化较大，且未观察到微量白蛋白尿浓度与尿IgG之间的相关性（r = 0.02）。尿IgG的检测作为糖尿病肾病的早期诊断方法并无用处。
