Zhang Z, Schlachta C, Duff J, Stiller C, Grant D, Zhong R
Department of Surgery and Medicine, University of Western Ontario, London, Canada.
Microsurgery. 1995;16(2):103-9. doi: 10.1002/micr.1920160212.
Improved microsurgical techniques for kidney transplantation in the mouse are described. Left renal transplantation is performed with end-to-side anastomoses of the donor renal vein to the inferior vena cava and the donor aortic cuff to the aorta. Urinary tract reconstruction is accomplished by suturing the donor ureter with a bladder patch to the recipient bladder. With these modifications, it is possible to achieve success rates as high as 90%. This stable and reproducible model provides a useful tool to study the immunological mechanisms of kidney allograft rejection at the molecular level.
本文描述了用于小鼠肾移植的改良显微外科技术。左肾移植采用供体肾静脉与下腔静脉端侧吻合，以及供体主动脉袖片与主动脉端侧吻合。尿路重建是通过将带有膀胱补片的供体输尿管缝合到受体膀胱来完成的。通过这些改良，成功率可达90%。这个稳定且可重复的模型为在分子水平研究同种异体肾移植排斥反应的免疫机制提供了一个有用的工具。
