Santos F X, Bilbao G, Rodrigo J, Fernández J, Martínez D, Mayoral E, Rodríguez J
Experimental Surgery Unit, Hospital Universitario del Aire, Madrid, Spain.
Microsurgery. 1995;16(2):71-6. doi: 10.1002/micr.1920160206.
An experimental model for local administration of neuronotrophic substances at the site of peripheral nerve lesion is presented. The model consists of a subcutaneously located silicone reservoir and a connecting tube with its distal end fixed in the proximity of the severed and repaired nerve. The results of the preliminary tests of the model are presented. Sixty Sprague-Dawley rats were divided into two groups: control (saline-treated) (n = 30) and NGF-treated (n = 30). After axotomy of the sciatic nerve, an epineurial repair is performed. NGF or saline is injected daily into the subcutaneous reservoir during the first 3 weeks after axotomy and a single dose in the fourth week. The regenerated nerve observed in the NGF-treated group after four weeks of treatment presents a greater percentage of myelinated axons, thicker myeline sheaths, and more mature endoneurial layers. This experimental model provides a reliable and quantitative way to deliver neuronotrophic substances in site and at different administration rates.
本文介绍了一种用于在周围神经损伤部位局部施用神经营养物质的实验模型。该模型由一个皮下放置的硅胶储液器和一根连接管组成，连接管的远端固定在切断并修复的神经附近。文中给出了该模型的初步测试结果。将60只Sprague-Dawley大鼠分为两组：对照组（生理盐水处理）（n = 30）和NGF处理组（n = 30）。坐骨神经切断术后，进行神经外膜修复。在切断术后的前三周，每天将NGF或生理盐水注入皮下储液器，第四周注射单剂量。治疗四周后，在NGF处理组中观察到的再生神经呈现出更高比例的有髓轴突、更厚的髓鞘和更成熟的神经内膜层。该实验模型提供了一种可靠且定量的方法，可在局部以不同的给药速率递送神经营养物质。
