在普通脱硫弧菌（宫崎F株）中，一个编码细胞色素c氧化酶样蛋白的基因与细胞色素c-553基因紧密相邻。

A gene encoding a cytochrome c oxidase-like protein is located closely to the cytochrome c-553 gene in the anaerobic bacterium, Desulfovibrio vulgaris (Miyazaki F).

作者信息

Kitamura M, Mizugai K, Taniguchi M, Akutsu H, Kumagai I, Nakaya T

机构信息

Department of Bioapplied Chemistry, Faculty of Engineering, Osaka City University, Japan.

出版信息

Microbiol Immunol. 1995;39(1):75-80. doi: 10.1111/j.1348-0421.1995.tb02172.x.

DOI:10.1111/j.1348-0421.1995.tb02172.x

PMID:7783682

Abstract

The gene encoding cytochrome c-553 from Desulfovibrio vulgaris (Miyazaki F) was cloned using a synthetic oligodeoxyribonucleotide probe. The nucleotide sequence indicated that cytochrome c-553 was synthesized as a precursor protein with an NH2-terminal signal sequence of 23 residues. In the cloned DNA fragment, there are three other open reading frames whose products have 191, 157, 541 amino acid residues, respectively. The putative ORF-4 product is highly homologous with the cytochrome c oxidase subunit I from various organisms.

摘要

利用合成的寡聚脱氧核糖核苷酸探针克隆了来自普通脱硫弧菌（宫崎F株）的细胞色素c-553编码基因。核苷酸序列表明细胞色素c-553作为一种前体蛋白被合成，其氨基末端有一个23个残基的信号序列。在克隆的DNA片段中，还有另外三个开放阅读框，其产物分别有191、157、541个氨基酸残基。推测的ORF-4产物与来自各种生物体的细胞色素c氧化酶亚基I高度同源。