Belov G A, Gmyl' A P, Maslova S V, Pilipenko E V, Agol V I
Mol Biol (Mosk). 1995 Mar-Apr;29(2):294-300.
Translation of polioviral RNA is initiated by interaction of a small ribosomal subunit with internal segments of the 5'-untranslated region (5'UTR). Several mutations were constructed within 5'UTR segment 425-449. All of them (including a single C444-->U replacement) inhibited in vitro translation, which decreased about 10-fold. Two mutant constructs, pPV12-05 (C444-->U) and pPV12K (containing also an AAUU insert between positions 441 and 442) produced plaques on monolayers of susceptible cells. All the viruses isolated from these plaques exhibited a reversion at position 444; the template activities of the revertant RNAs were restored completely or significantly. The results show the importance of the relevant 5'UTR segment for the initiation of polioviral RNA translation.
脊髓灰质炎病毒RNA的翻译起始于小核糖体亚基与5'非翻译区（5'UTR）内部片段的相互作用。在5'UTR的425 - 449片段内构建了多个突变体。所有这些突变体（包括单个C444→U替换）均抑制体外翻译，翻译效率下降约10倍。两个突变体构建体，pPV12 - 05（C444→U）和pPV12K（在441和442位之间还含有AAUU插入序列）在易感细胞单层上产生噬斑。从这些噬斑中分离出的所有病毒在444位均出现回复突变；回复突变RNA的模板活性完全或显著恢复。结果表明相关的5'UTR片段对脊髓灰质炎病毒RNA翻译起始的重要性。
