[Production of IFN gamma, IL6 and IL10 by infiltrating cells of renal allografts and irreversible rejection: modulation by IL2 and IL4].

The frequency of isolated low density graft-infiltrating cells (GIC) secreting IFN gamma, IL6 and IL10 was studied in 8 cases of irreversible rejection of human renal allografts, using ELISPOT assays. The GIC were mostly CD8+ T cells although CD4+ T cells, B cells and macrophages could also be detected. In average, in 10(6) cells, 189 secreted IFN gamma, 747 IL10, and 17,114 IL6. Culture of GIC in the presence of IL2 resulted in an increase on the frequency of IFN gamma producing cells (IFN gamma-PC), in a dose dependent manner, with an optimal 6.5 fold increase at 50 U/ml. In contrast, 25 U/ml IL4 decreased the frequency of spontaneous and IL2 induced IFN gamma-PC. Furthermore, IL4 reduced the frequency of IL6-PC.