González-y-Merchand J, Ruiloba de León S, Estrada-García I, López-Flores C, Ortega-Ortega R, Ruíz-Puente J
Departamento de Microbiología e Inmunología, Escuela Nacional de Ciencias Biológicas, México, D.F., Mexico.
Rev Latinoam Microbiol. 1995 Jan-Mar;37(1):33-41.
In this work the biological properties and the capability of the outer membrane proteins (OMPs) from different strains of Bordetella to induce protection against challenge with B. pertussis 18323 were examined. The OMPs from each strain were isolated using Schnaitmann's method. Two OMPs (30 and 32 kDa) were found to be specific for the vaccine strains of B. pertussis and were absent in the OMPs preparation from both B. parapertussis and B. bronchiseptica. When the OMPs from the vaccine strains of B. pertussis were assayed in the mouse intracerebral protection test, they were found to be highly protective (75%-88%) against a challenge with 250 50% lethal doses (LD50) of B. pertussis 18323. However, no correlation was observed between the protective activity and the lymphocytosis-promoting factor (LPF) content of different preparations. Moreover, neither LPF activity or histamine-sensitizing activity (HSA) were found in any of the OMPs assayed. Our results show that OMPs from B. pertussis vaccine strains play a key role in the induction of protective immunity against B. pertussis 18323 in mice, making them excellent candidates to be used in further studies for the development of a pertussis vaccine for humans.
在本研究中，检测了不同博德特氏菌菌株外膜蛋白（OMPs）的生物学特性以及诱导针对百日咳博德特氏菌18323攻击的保护能力。使用施奈特曼方法分离各菌株的OMPs。发现两种OMPs（30 kDa和32 kDa）是百日咳博德特氏菌疫苗菌株特有的，而副百日咳博德特氏菌和支气管败血博德特氏菌的OMPs制剂中不存在。当在小鼠脑内保护试验中检测百日咳博德特氏菌疫苗菌株的OMPs时，发现它们对250个50%致死剂量（LD50）的百日咳博德特氏菌18323攻击具有高度保护作用（75%-88%）。然而，不同制剂的保护活性与淋巴细胞增多促进因子（LPF）含量之间未观察到相关性。此外，在所检测的任何OMPs中均未发现LPF活性或组胺致敏活性（HSA）。我们的结果表明，百日咳博德特氏菌疫苗菌株的OMPs在诱导小鼠针对百日咳博德特氏菌18323的保护性免疫中起关键作用，使其成为进一步研究开发人类百日咳疫苗的优秀候选物。
