Benov L, Chang L Y, Day B, Fridovich I
Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA.
Arch Biochem Biophys. 1995 Jun 1;319(2):508-11. doi: 10.1006/abbi.1995.1324.
Cu,ZnSOD purified from Escherichia coli has been used to raise antibodies in rabbits. The resultant antiserum was found to recognize a single band on Western blots of SDS-polyacrylamide gel electropherograms, and that single band coincided with the position of the Cu,ZnSOD. Ultrathin sections of fixed E. coli were treated with the antibody followed by protein A bearing 10-nm gold particles. Electron microscopy revealed that Cu,ZnSOD was largely localized in the periplasm in polar bays.
从大肠杆菌中纯化得到的铜锌超氧化物歧化酶（Cu,ZnSOD）已被用于在兔子体内产生抗体。结果发现，所得抗血清在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱的蛋白质免疫印迹上识别出一条单一的条带，且该单一条带与铜锌超氧化物歧化酶的位置一致。将固定后的大肠杆菌超薄切片用该抗体处理，随后用带有10纳米金颗粒的蛋白A处理。电子显微镜显示，铜锌超氧化物歧化酶主要定位于极性区域的周质中。
