Means T L, Geroski D H, Hadley A, Lynn M J, Edelhauser H F
Department of Ophthalmology, School of Medicine, Emory University, Atlanta, Ga., USA.
Arch Ophthalmol. 1995 Jun;113(6):805-9. doi: 10.1001/archopht.1995.01100060131047.
To evaluate endothelial viability of human corneas stored in glass vials and in viewing chambers (Alcon) for extended periods, and to compare endothelial viability of Optisol-GS-stored corneas with corneas excised from moist chamber-stored globes.
Endothelial viability was assessed using two staining techniques. Endothelium from stored corneas was stained with trypan blue combined with alizarin red S or stained with calcein AM-ethidium homodimer. Both techniques were used to determine which method is a more sensitive indicator of cytotoxic change.
Corneas stored 4 to 21 days in Optisol-GS had a rate (mean +/- SE) of endothelial cell damage of 0.57% +/- 0.30% per day in vials and 0.69% +/- 0.27% in chambers. After storage intervals from 4 to 21 days, the Optisol-GS endothelium had an average decrease in viability of 9.5% to 16%. The endothelium of moist chamber eyes had a 44% to 59% decrease in viability after 2 to 5 days. After 24 hours, corneal endothelium of moist chamber eyes had less than 15% decrease in viability. Optisol-GS corneas stored for 35 to 56 days had greater than 50% decrease in endothelial viability. After 67 days, 95% to 100% of endothelial viability was lost.
Corneas stored in Optisol-GS through 21 days at 4 degrees C maintain a high percentage of viable endothelial cells. There was no significant difference of endothelial viability between corneas stored in glass vials or in viewing chambers (Alcon). A 50% loss of endothelial viability occurred in moist chamber-stored corneas after 2 days and by 35 days in corneas stored in Optisol-GS.
评估长时间保存在玻璃瓶和观察室（爱尔康公司）中的人角膜的内皮细胞活力，并比较Optisol - GS保存的角膜与从湿润房保存的眼球中切除的角膜的内皮细胞活力。
使用两种染色技术评估内皮细胞活力。将保存角膜的内皮用台盼蓝与茜素红S联合染色或用钙黄绿素AM - 溴化乙锭同二聚体染色。两种技术均用于确定哪种方法是细胞毒性变化更敏感的指标。
在Optisol - GS中于4℃保存4至21天的角膜，在玻璃瓶中内皮细胞损伤率（均值±标准误）为每天0.57%±0.30%，在观察室中为0.69%±0.27%。在4至21天的保存期后，Optisol - GS中的角膜内皮活力平均下降9.5%至16%。湿润房眼中的角膜内皮活力在2至5天后下降44%至59%。24小时后，湿润房眼中的角膜内皮活力下降不到15%。在Optisol - GS中保存35至56天的角膜内皮活力下降超过50%。67天后，95%至100%的内皮活力丧失。
在4℃下于Optisol - GS中保存21天的角膜维持了高比例的存活内皮细胞。保存在玻璃瓶或观察室（爱尔康公司）中的角膜内皮活力无显著差异。湿润房保存的角膜在2天后内皮活力丧失50%，而在Optisol - GS中保存的角膜在35天后出现这种情况。
