肌球蛋白横桥的工作行程。
The working stroke of myosin crossbridges.
作者信息
Huxley H
机构信息
Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254, USA.
出版信息
Biophys J. 1995 Apr;68(4 Suppl):55S-56S; discussion 57S-58S.
Abstract
Recent x-ray diffraction measurements of the axial periodicities of the actin and myosin filaments in contracting muscles show that they are stretched by small but significant amounts by the developed tension, so that at least one half, possibly more, of the observed compliance of a sarcomere must reside in the filaments themselves. This implies that the movement steps of a crossbridge deduced from quick-release experiments may be shorter than some previous estimates, necessitating at least two steps to account for current in vitro single-molecule measurements. Intensity measurements of the wider angle x-ray reflections also show some unexpected features.
摘要
最近对收缩肌肉中肌动蛋白丝和肌球蛋白丝轴向周期性的X射线衍射测量表明，它们会因产生的张力而被拉伸，拉伸量虽小但很显著，因此，肌节观察到的顺应性中至少有一半（可能更多）必定存在于肌丝本身。这意味着，从快速释放实验推断出的横桥运动步长可能比之前的一些估计要短，这使得至少需要两步才能解释当前的体外单分子测量结果。广角X射线反射的强度测量也显示出一些意想不到的特征。
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本文引用的文献
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Nature. 1994 Mar 10;368(6467):113-9. doi: 10.1038/368113a0.
2
Single-molecule analysis of the actomyosin motor using nano-manipulation.使用纳米操纵对肌动球蛋白马达进行单分子分析。
Biochem Biophys Res Commun. 1994 Mar 15;199(2):1057-63. doi: 10.1006/bbrc.1994.1336.
3
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5
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6
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7
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