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The shapes of the motor domains of two oppositely directed microtubule motors, ncd and kinesin: a neutron scattering study.两种反向微管马达蛋白ncd和驱动蛋白的马达结构域形状：一项中子散射研究
Biophys J. 1995 Oct;69(4):1563-8. doi: 10.1016/S0006-3495(95)80028-7.

本文引用的文献

1
Structural and functional domains of the Drosophila ncd microtubule motor protein.果蝇ncd微管运动蛋白的结构域和功能域
J Biol Chem. 1993 Apr 25;268(12):9005-13.
2
Myosin-ATP chemomechanics.肌球蛋白 - ATP 化学力学
Biochemistry. 1993 Mar 16;32(10):2455-8. doi: 10.1021/bi00061a001.
3
Three-dimensional structure of myosin subfragment-1: a molecular motor.肌球蛋白亚片段-1的三维结构：一种分子马达。
Science. 1993 Jul 2;261(5117):50-8. doi: 10.1126/science.8316857.
4
Tubulin GTP hydrolysis influences the structure, mechanical properties, and kinesin-driven transport of microtubules.微管蛋白的鸟苷三磷酸水解作用会影响微管的结构、力学性质以及驱动蛋白介导的微管运输。
J Biol Chem. 1994 Sep 23;269(38):23769-75.
5
Implications of diffusion-controlled limit for processivity of dimeric kinesin head domains.扩散控制极限对二聚体驱动蛋白头部结构域持续运动性的影响。
Biophys J. 1995 Apr;68(4 Suppl):267S-269S; discussion 269S-270S.
6
Myosin subfragment 1 has tertiary structural domains.肌球蛋白亚片段1具有三级结构域。
Biochemistry. 1986 Apr 22;25(8):2237-42. doi: 10.1021/bi00356a058.
7
Birefringence of macromolecules. Wiener's theory revisited, with applications to DNA and tobacco mosaic virus.大分子的双折射。重温维纳理论及其在DNA和烟草花叶病毒中的应用。
Biophys J. 1989 Jul;56(1):195-205. doi: 10.1016/S0006-3495(89)82664-5.
8
Characterization of the ethenoadenosine diphosphate binding site of myosin subfragment 1. Energetics of the equilibrium between two states of nucleotide.S1 and vanadate-induced global conformation changes detected by energy transfer.
Biochemistry. 1989 Jan 24;28(2):799-807. doi: 10.1021/bi00428a058.
9
Ligand-induced myosin subfragment 1 global conformational change.配体诱导的肌球蛋白亚片段1整体构象变化。
Biochemistry. 1990 May 1;29(17):4087-93. doi: 10.1021/bi00469a010.
10
Identification and characterization of a gene encoding a kinesin-like protein in Drosophila.果蝇中一种编码类驱动蛋白的基因的鉴定与表征
Cell. 1990 Jun 15;61(6):991-1000. doi: 10.1016/0092-8674(90)90064-l.

两个分子马达结构域的溶液结构：非红葡萄酒分离蛋白和驱动蛋白。

Solution structure of two molecular motor domains: nonclaret disjunctional and kinesin.

作者信息

Eden D, Luu B Q, Zapata D J, Sablin E P, Kull F J

机构信息

Department of Chemistry and Biochemistry, San Francisco State University 94132, USA.

出版信息

Biophys J. 1995 Apr;68(4 Suppl):59S-64S; discussion 65S.

PMID:7787102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1281867/
Abstract

The effects of selected ligands on the structure of the truncated heavy-chain chemomechanical motor domains of Drosophila ncd and human kinesin were compared using the technique of transient electric birefringence. The 366-amino acid C-terminal motor domain of Drosophila nonclaret disjunctional, ncd(335-700), and the 349-amino acid N-terminal motor domain of human kinesin, kinesin(349), were studied at 4 degrees C in neutral buffers with ionic strength of 100 mM to form complexes with either MgADP or MgADP.Vi. The rotational diffusion time adjusted to 20 degrees C and water, tau 20,W, for ncd(335-700).MgADP is 32.8 ns, and for ncd(335-700).MgADP.Vi is 34.8 ns, suggesting prolate ellipsoids with dimensions 9.40 x 3.77 nm and 9.73 x 3.70 nm, respectively. The specific Kerr constant, Ksp, of ncd is -1.65 x 10(-12) cm2V-2 for the MgADP complex and -1.15 x 10(-12) cm2V-2 for the MgADP.Vi complex. The large negative Ksp for a prolate protein suggests an unusual charge distribution with two long surfaces with opposite charge. The tau 20,W for kinesin(349).MgADP is longer than the corresponding ncd motor and shows a decrease with increased electric field. The kinesin(349).MgADP.Vi complex has a longer tau 20,W. The Ksp for kinesin(349) is 0.36 x 10(-12) cm2V-2 for each complex.

摘要

使用瞬态电双折射技术比较了选定配体对果蝇ncd和人驱动蛋白截短重链化学机械运动结构域结构的影响。在4℃、离子强度为100 mM的中性缓冲液中研究了果蝇非清晰分离蛋白（ncd）的366个氨基酸的C末端运动结构域ncd(335 - 700)和人驱动蛋白的349个氨基酸的N末端运动结构域kinesin(349)，使其与MgADP或MgADP·Vi形成复合物。将ncd(335 - 700)·MgADP在20℃和水中的旋转扩散时间调整后，τ20,W为32.8 ns，ncd(335 - 700)·MgADP·Vi的为34.8 ns，表明分别为尺寸为9.40×3.77 nm和9.73×3.70 nm的长椭球体。ncd与MgADP复合物的比克尔常数Ksp为 -1.65×1( -12) cm2V -2，与MgADP·Vi复合物的为 -1.15×10( -12) cm2V -2。长形蛋白质的大负Ksp表明电荷分布异常，有两个带相反电荷的长表面。kinesin(349)·MgADP的τ20,W比相应的ncd运动结构域长，并且随电场增加而减小。kinesin(349)·MgADP·Vi复合物的τ20,W更长。kinesin(349)每种复合物的Ksp为0.36×10( -12) cm2V -2。