Prändl R, Kloske E, Schöffl F
Universität Tübingen, Biologisches Institut, Germany.
Plant Mol Biol. 1995 Apr;28(1):73-82. doi: 10.1007/BF00042039.
The heat shock (hs) response during plant growth and development was analyzed in tobacco and Arabidopsis using chimaeric beta-glucuronidase reporter genes (hs-Gus) driven by a soybean hs promoter. Fluorimetric measurements and histochemical staining revealed high Gus activities in leaves, roots, and flowers exclusively after heat stress. The highest levels of heat-inducible expression were found in the vascular tissues. Without heat stress, a developmental induction of hs-Gus was indicated by the accumulation of high levels of Gus in transgenic tobacco seeds. There was no developmental induction of hs-Gus in Arabidopsis seeds. In situ hybridization to the RNA of the small heat shock protein gene Athsp17.6 in tissue sections revealed an expression in heat-shocked leaves but no expression in control leaves of Arabidopsis. However, a high level of constitutive expression of hs genes was detected in meristematic and provascular tissues of the Arabidopsis embryo. The developmental and tissue-specific regulation of the hs response is discussed.
利用由大豆热激启动子驱动的嵌合β-葡萄糖醛酸酶报告基因（hs-Gus），在烟草和拟南芥中分析了植物生长发育过程中的热激（hs）反应。荧光测量和组织化学染色显示，仅在热胁迫后，叶片、根和花中才有较高的Gus活性。在维管组织中发现了最高水平的热诱导表达。在没有热胁迫的情况下，转基因烟草种子中高水平Gus的积累表明了hs-Gus的发育诱导。拟南芥种子中没有hs-Gus的发育诱导。对组织切片中小热激蛋白基因Athsp17.6的RNA进行原位杂交，结果显示在热激的拟南芥叶片中有表达，但在对照叶片中无表达。然而，在拟南芥胚胎的分生组织和原维管组织中检测到hs基因的高水平组成型表达。本文讨论了hs反应的发育和组织特异性调控。
