Taub R N, Schanzer H, Bramis J
Proc Eur Dial Transplant Assoc. 1976;12:423-8.
Spleens from Lewis X BN F1 hybrid rats were directly anastamosed to the circulation of parental strain Lewis rats by a microvascular technique; no immunosuppressive drugs were given. Donor spleens enlarged massively during the first month after implantation, then grew gradually smaller over succeeding months without compromising their blood supply. Renal allografts were placed into bilaterally nephrectomised recipients that had already borne a splenic allograft for three months. The grafts functioned perfectly and showed no signs of rejection five months later. Donor LBN skin allografts transplanted to these animals showed markedly prolonged survival while third-party BUF rat skin allografts were rejected in first-set fashion. High titres of anti-LBN haemagglutinating antibody could be detected in the circulation of spleen-grafted animals during the first month; the titres fell to zero by the fourth month. Cellular reactivity of recipient cells against LBN antigens measured by the popliteal node GVH assay fell to minimal levels by the third month. These data indicate that the immune response to allografted tissues may be specifically and markedly suppressed in adult animals by continued presence of a donor-strain spleen.
通过微血管技术将Lewis X BN F1杂交大鼠的脾脏直接吻合到亲本品系Lewis大鼠的循环系统中；未给予免疫抑制药物。供体脾脏在植入后的第一个月内大量增大，随后在接下来的几个月中逐渐变小，但不影响其血液供应。将肾同种异体移植物植入双侧肾切除的受体中，这些受体已经接受脾同种异体移植三个月。移植物功能良好，五个月后未出现排斥迹象。移植到这些动物身上的供体LBN皮肤同种异体移植物存活时间明显延长，而第三方BUF大鼠皮肤同种异体移植物则以初次排斥的方式被排斥。在第一个月内，可在脾移植动物的循环系统中检测到高滴度的抗LBN血凝抗体；到第四个月时，滴度降至零。通过腘窝淋巴结GVH试验测量，受体细胞对LBN抗原的细胞反应性到第三个月降至最低水平。这些数据表明，成年动物中供体品系脾脏的持续存在可能会特异性且显著地抑制对同种异体移植组织的免疫反应。
