瘦猪和肥胖猪血清对培养的猪脂肪基质血管细胞分化的影响。

Effect of sera from lean and obese pigs on the differentiation of porcine adipose stromal-vascular cells in culture.

作者信息

Akanbi K A, Hu C Y

机构信息

Department of Animal Sciences, Oregon State University, Corvallis 97331, USA.

出版信息

Comp Biochem Physiol A Physiol. 1995 Jun;111(2):293-8. doi: 10.1016/0300-9629(94)00212-c.

DOI:10.1016/0300-9629(94)00212-c

PMID:7788352

Abstract

Primary cultures of stromal-vascular (S-V) cells from adipose tissue of new-born pigs were used to evaluate the characteristics of four sera obtained from male and female genetically lean or obese pigs weighing 50-60 kg. (2) Lean pig sera (LPS) stimulated greater levels of sn-glycerol-3-phosphate dehydrogenase (GPDH) activity in cultured cells than obese sera (OPS). Male LPS tended to promote higher GPDH specific activity than female LPS or male and female OPS. When GPDH was expressed on a per DNA basis, male LPS significantly (P < 0.05) increased GPDH per unit DNA compared with female LPS or male and female OPS.

摘要

使用新生猪脂肪组织的基质血管（S-V）细胞原代培养物来评估从体重50-60千克的雄性和雌性基因瘦型或肥胖型猪获得的四种血清的特性。（2）与肥胖血清（OPS）相比，瘦猪血清（LPS）刺激培养细胞中sn-甘油-3-磷酸脱氢酶（GPDH）活性的水平更高。雄性LPS比雌性LPS或雄性和雌性OPS倾向于促进更高的GPDH比活性。当基于每个DNA来表达GPDH时，与雌性LPS或雄性和雌性OPS相比，雄性LPS显著（P <0.05）增加了每单位DNA的GPDH。