[Preparation and characterization of monoclonal antibodies against esterase of malathion-resistant Culex quinquefasciatus].

Five clones of monoclonal antibodies (McAbs) were generated from BALB/c mice which were activized with PPD and immunized with esterases extracted from malathion-resistant Culex quinquefasciatus. All 5 clones of McAbs belonged to the IgG1 subtype, additive index (A.I.) of every two McAbs did not exceeded 50%. After a 1 h of incubation, the McAbs at the titer of 1:1,000 completely inhibited the activity of the amplified esterases. Of 8 strains of adult mosquitoes studied by Western-blot assay, all exhibited the 64 kDa esterase band recognized by the McAbs, six (SP, FS, SH, CD, RD, RM) showed more bands of different MW (43-60 kDa), the higher the organophosphorus resistant level, the more and the denser the bands. The above McAbs had little or no effect against the esterases of dimethrin-resistant strain and the larva stage of malathion-resistant Culex quinquefasciatus.