Nazni W A, Lee H L, Sa'diyah I
Division of Medical Entomology, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
Southeast Asian J Trop Med Public Health. 1998 Dec;29(4):849-55.
Wild caught female Culex quinquefasciatus (Say) from Kuala Lumpur were blood fed and reared in the insectarium. The late third stage of the F1 larvae which survived the high selection pressure of malathion and permethrin were reared and colonies were established from adults that emerged. Larvae from these colonies were then subjected in the subsequent 9 generations to higher selection pressure. The rate of resistance development were measured by LC50 value of larval bioassay, LT50 value of adult bioassay and the frequency of the elevated esterase levels. In another set of experiments using the same batch of Culex mosquitos, the larvae were not exposed to any insecticides and the decrease in resistance rate was monitored in each subsequent 9 generations by using similar methods. The heterozygous standard laboratory strain was selected for susceptibility using the single raft sib-selection method. The result showed that the field collected F1 generation was 96.0 and 6.3 fold more resistant to malathion and permethrin, respectively. After selection for about 9 generations the resistance ratio to malathion and permethrin was 6.2 and 767.3 fold more compared to the LC50 values of F1 generations, respectively. Esterase in F1 larvae was 6.0 fold more than the standard laboratory strain.
从吉隆坡野外捕获的雌性致倦库蚊（Say）进行血饲后，在昆虫饲养室饲养。在马拉硫磷和氯菊酯的高选择压力下存活下来的F1代幼虫的晚期第三阶段被饲养，并从羽化的成虫中建立种群。然后，这些种群的幼虫在随后的9代中受到更高的选择压力。通过幼虫生物测定的LC50值、成虫生物测定的LT50值和酯酶水平升高的频率来测量抗性发展速率。在另一组使用同一批库蚊的实验中，幼虫不接触任何杀虫剂，并使用类似方法在随后的每9代中监测抗性率的下降。使用单筏同胞选择法选择杂合标准实验室品系以测定其敏感性。结果表明，野外采集的F1代对马拉硫磷和氯菊酯的抗性分别是原来的96.0倍和6.3倍。经过约9代的选择后，与F1代的LC50值相比，对马拉硫磷和氯菊酯的抗性比分别提高了6.2倍和767.3倍。F1代幼虫中的酯酶比标准实验室品系高6.0倍。
