Salam M A, Crocker J, Morris A
Department of Otolaryngology, Queen Elizabeth University Hospital, Birmingham, UK.
Clin Otolaryngol Allied Sci. 1995 Feb;20(1):49-52. doi: 10.1111/j.1365-2273.1995.tb00011.x.
p53 is a nuclear phosphoprotein which acts as a tumour suppressor factor, regulating cell growth and division. Mutations in the p53 gene appear to be the most common genetic alterations in human cancer. The aim of this study was to investigate p53 expression in laryngeal squamous cell carcinomas and to assess its role as a marker of prognostic significance. Using immunohistochemical staining techniques, a series of laryngeal carcinomas (n = 87) were examined for expression of the mutant form of p53 phosphoprotein using the monoclonal antibody PAB 1801. p53 over-expression was noted in 50 biopsies of laryngeal carcinomas (57.5%) but not in any of the non-neoplastic laryngeal mucosa which were used as the control. There was no statistical correlation between p53 immunoreactivity and the clinicopathological parameters of the cancers including: site of tumour, TNM staging, differentiation grading and tumour recurrence. These findings indicate that p53 expression is strongly associated with carcinoma cells and not with normal cells in the larynx. However, p53 expression is probably unrelated to the biological aggressiveness of these tumours.
p53是一种核磷蛋白，作为肿瘤抑制因子发挥作用，调节细胞生长和分裂。p53基因的突变似乎是人类癌症中最常见的基因改变。本研究的目的是调查p53在喉鳞状细胞癌中的表达，并评估其作为具有预后意义标志物的作用。使用免疫组织化学染色技术，采用单克隆抗体PAB 1801对一系列喉癌（n = 87）进行p53磷蛋白突变形式的表达检测。在50例喉癌活检组织（57.5%）中发现p53过表达，但在用作对照的任何非肿瘤性喉黏膜中均未发现。p53免疫反应性与癌症的临床病理参数之间无统计学相关性，这些参数包括：肿瘤部位、TNM分期、分化分级和肿瘤复发。这些发现表明，p53表达与癌细胞密切相关，而与喉中的正常细胞无关。然而，p53表达可能与这些肿瘤的生物学侵袭性无关。
