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建立抗Yo抗体介导的副肿瘤性小脑变性动物模型的实验。1. 携带不同主要组织相容性复合体(MHC)分子的小鼠品系在用重组Yo蛋白免疫后会产生抗体,但不会导致浦肯野细胞丢失。

Trial to establish an animal model of paraneoplastic cerebellar degeneration with anti-Yo antibody. 1. Mouse strains bearing different MHC molecules produce antibodies on immunization with recombinant Yo protein, but do not cause Purkinje cell loss.

作者信息

Tanaka M, Tanaka K, Onodera O, Tsuji S

机构信息

Department of Neurology, Niigata University, Japan.

出版信息

Clin Neurol Neurosurg. 1995 Feb;97(1):95-100. doi: 10.1016/0303-8467(95)00005-5.

Abstract

Passive transfer of anti-Yo antibody from patients with paraneoplastic cerebellar degeneration (PCD) associated with gynecological or breast carcinoma has not been successful in inducing an animal model. We used active immunization with recombinant Yo protein of four strains of mice bearing different MHC molecules: BALB/c (H-2d), C3H (H-2k), C57BL/6 (H-2b), SJL/J (H-2s). All the strains produced high anti-Yo antibody titer but none developed cerebellar ataxia or showed Purkinje cell loss. Spleen cells from the immunized mice also reacted with recombinant protein. Because C57BL/6(nu/nu) mice produce no anti-Yo antibody, mature helper T cells are required for its production. Results suggest that antibody production in peripheral blood alone is not sufficient for the development of PCD and that MHC class II molecules function in the activation of T cells to help B cells to help B cells produce antibodies.

摘要

将患有与妇科或乳腺癌相关的副肿瘤性小脑变性(PCD)患者的抗Yo抗体进行被动转移,未能成功诱导出动物模型。我们用重组Yo蛋白对四种携带不同MHC分子的小鼠品系进行主动免疫:BALB/c(H-2d)、C3H(H-2k)、C57BL/6(H-2b)、SJL/J(H-2s)。所有品系均产生了高抗Yo抗体滴度,但无一出现小脑共济失调或浦肯野细胞丢失。免疫小鼠脾细胞也与重组蛋白发生反应。由于C57BL/6(nu/nu)小鼠不产生抗Yo抗体,因此其产生需要成熟的辅助性T细胞。结果表明,仅外周血中的抗体产生不足以引发PCD,且MHC II类分子在激活T细胞以帮助B细胞产生抗体的过程中发挥作用。

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