Tanaka K, Tanaka M, Onodera O, Igarashi S, Tsuji S
Department of Neurology, Niigata University, Japan.
No To Shinkei. 1994 Jan;46(1):47-51.
Paraneoplastic cerebellar degeneration (PCD) is a remote effect of cancer mediated by possibly immunological mechanisms. The sera and cerebrospinal fluid of PCD patients containing high titer autoantibody against cerebellar Purkinje cells had been reported. This antibody binds to 62-kD and 34-kD bands of cerebellar Purkinje cell homogenates (anti-Yo antibody). However, it is not always true that the autoantibody of this character on immunoblot and immunohistochemistry recognizes the same molecule. Recently, the DNA sequence encoding the Yo antigen, whose common epitope is a sequence containing leucine-zipper motif, was reported. We made the recombinant protein deduced from the cDNA clone encoding the leucine-zipper motif of the Yo antigen. Using this recombinant protein as the antigen for ELISA, the anti Yo antibodies in the sera and CSF were examined and 3 new patients with PCD possessing anti-Yo antibody were found. The sera of one patient, serially taken during several kinds of treatment were examined with this ELISA system, which revealed that the anti-Yo antibody titer was increased after plasmapheresis and reduced after tumor resection and anti-cancer chemotherapy. The early resection of malignant tumors may prevent the continuous production of high titer anti-Yo antibody and stop the progression of cerebellar tissue damage.
副肿瘤性小脑变性（PCD）是一种可能由免疫机制介导的癌症远隔效应。已有报道称，PCD患者的血清和脑脊液中含有针对小脑浦肯野细胞的高滴度自身抗体。该抗体与小脑浦肯野细胞匀浆的62-kD和34-kD条带结合（抗Yo抗体）。然而，免疫印迹和免疫组化中具有这种特性的自身抗体并不总是识别同一分子。最近，报道了编码Yo抗原的DNA序列，其共同表位是一个包含亮氨酸拉链基序的序列。我们制备了由编码Yo抗原亮氨酸拉链基序的cDNA克隆推导的重组蛋白。用该重组蛋白作为ELISA的抗原，检测血清和脑脊液中的抗Yo抗体，发现3例新的具有抗Yo抗体的PCD患者。用该ELISA系统检测了1例患者在几种治疗期间连续采集的血清，结果显示血浆置换后抗Yo抗体滴度升高，肿瘤切除和抗癌化疗后降低。早期切除恶性肿瘤可能会阻止高滴度抗Yo抗体的持续产生，并阻止小脑组织损伤的进展。
