猪促卵泡素β亚基（FSHB）和促黄体素β亚基（LHB）基因的聚合酶链反应（PCR）扩增及物理定位

PCR amplification and physical localization of the genes for pig FSHB and LHB.

作者信息

Mellink C, Lahbib-Mansais Y, Yerle M, Gellin J

机构信息

Laboratoire de Génétique Cellulaire, Centre INRA-Toulouse, Castanet-Tolosan, France.

出版信息

Cytogenet Cell Genet. 1995;70(3-4):224-7. doi: 10.1159/000134039.

DOI:10.1159/000134039

PMID:7789177

Abstract

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are members of the glycoprotein hormone family and play essential roles in gametogenesis and sexual development of mammals. Fragments of the porcine genes coding for the beta subunits of FSH and LH were amplified by PCR and used as probes for radioactive in situ hybridization in order to map these loci in the pig. Primers were chosen on the genomic DNA nucleotide sequences of FSHB and LHB as published in GenBank. Fragments of 1,127 bp (FSHB) and 1,239 bp (LHB) were cloned and verified by sequencing. FSHB was localized to pig chromosome bands 2p1.6-->p1.2 and LHB to pig chromosome band 6q2.1. The localization of LHB to the so-called halothane region of chromosome 6 could be expected from comparative mapping data. For FSHB, no conclusions can be drawn in this respect since, up to the present, too few genes are located on porcine chromosome 2.

摘要

促卵泡激素（FSH）和促黄体生成素（LH）是糖蛋白激素家族的成员，在哺乳动物的配子发生和性发育中发挥着重要作用。通过聚合酶链反应（PCR）扩增编码猪FSH和LHβ亚基的基因片段，并将其用作放射性原位杂交的探针，以便在猪中定位这些基因座。根据GenBank中公布的FSHB和LHB基因组DNA核苷酸序列选择引物。克隆了1127bp（FSHB）和1239bp（LHB）的片段，并通过测序进行了验证。FSHB定位于猪染色体带2p1.6→p1.2，LHB定位于猪染色体带6q2.1。从比较图谱数据可以预期LHB定位于6号染色体的所谓氟烷区域。对于FSHB，由于目前位于猪2号染色体上的基因太少，在这方面无法得出结论。