Che J, Zhang J, Qu Z, Peng X
Department of Pharmacology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing.
Chin Med J (Engl). 1995 Apr;108(4):265-8.
Cytosolic free Ca2+ ([Ca2+]i) was measured in dissociated cerebral cells isolated from fetal rats with the fluorescent indicater fura-2. Increase in [Ca2+]i occurred rapidly following exposure of the cells to 50 mmol/L KCl, 10(-7) mol/L Bay K 8644 or 200 mumol/L glutamate (Glu). [Ca2+]i elevated by K(+)-depolarization was attenuated by pretreatment with 10(-7), 10(-6) mol/L daurisoline (Dau). The response of [Ca2+]i to K(+)-depolarization did not change when 10(-8) mol/L Dau was added. When 10(-8)-10(-6) mol/L Dau was added to the cell suspensions prior to exposure to Glu, the Glu-stimulated rises in [Ca2+]i were reduced significantly. However, Dau (10(-6), 10(-7) and 10(-8) mol/L) did not alter the response to Bay K 8644. These results indicate that Dau can inhibit the increase of [Ca2+]i in fetal rat cerebral cells induced by certain Ca2+ agonists, especially Glu, suggesting that this drug may have a protective effect against cerebral cellular injury.
采用荧光指示剂fura - 2测量从胎鼠分离的解离脑细胞中的胞质游离钙离子浓度（[Ca2+]i）。将细胞暴露于50 mmol/L氯化钾、10(-7) mol/L Bay K 8644或200 μmol/L谷氨酸（Glu）后，[Ca2+]i迅速升高。用10(-7)、10(-6) mol/L蝙蝠葛碱（Dau）预处理可减弱由钾离子去极化引起的[Ca2+]i升高。添加10(-8) mol/L Dau时，[Ca2+]i对钾离子去极化的反应未改变。在暴露于Glu之前，向细胞悬液中添加10(-8)-10(-6) mol/L Dau，Glu刺激引起的[Ca2+]i升高显著降低。然而，Dau（10(-6)、10(-7)和10(-8) mol/L）并未改变对Bay K 8644的反应。这些结果表明，Dau可抑制某些钙离子激动剂（尤其是Glu）诱导的胎鼠脑细胞中[Ca2+]i升高，提示该药物可能对脑损伤具有保护作用。
