Rider V, Piva M, Cohen M E, Carlone D L
Division of Molecular Biology and Biochemistry, University of Missouri-Kansas City, School of Biological Sciences 64110, USA.
Endocrinology. 1995 Jul;136(7):3137-45. doi: 10.1210/endo.136.7.7789341.
Recent studies suggest that steroid effects on uterine cell proliferation may be moderated by polypeptide growth factors. We now provide evidence that high affinity fibroblast growth factor (FGF) receptors are present temporally and spatially in the pregnant rat uterus (days 4-6) to support the idea that basic FGF action occurs via binding to its high affinity FGF receptor 1 (FGFR1). Reverse transcription-polymerase chain amplification indicates that both the full-length transcript and an alternatively spliced messenger RNA are present in the uterus. Western immunoblot analysis confirms that rat uterine membrane proteins contain two receptor isoforms, and these receptors bind basic FGF with high affinity and specificity. Immunolocalization of FGFR1 revealed receptor-positive cells in both the uterine stroma and epithelia on days 4-6 of pregnancy. However, the receptor was differentially localized in the disparate cell types. The nuclei of stromal cells were positive for FGFR1, whereas epithelial cell nuclei were negative. Together, these results suggest that FGF signal transduction in uterine stromal cells is mediated by activation of FGFR1.
近期研究表明，类固醇对子宫细胞增殖的影响可能受到多肽生长因子的调节。我们现在提供证据表明，高亲和力成纤维细胞生长因子（FGF）受体在妊娠大鼠子宫（第4 - 6天）中存在时空分布，以支持碱性FGF通过与其高亲和力FGF受体1（FGFR1）结合发挥作用的观点。逆转录 - 聚合酶链扩增表明，子宫中同时存在全长转录本和可变剪接的信使RNA。蛋白质免疫印迹分析证实，大鼠子宫膜蛋白含有两种受体亚型，并且这些受体以高亲和力和特异性结合碱性FGF。FGFR1的免疫定位显示，在妊娠第4 - 6天，子宫基质和上皮中均有受体阳性细胞。然而，该受体在不同细胞类型中的定位存在差异。基质细胞核FGFR1呈阳性，而上皮细胞核呈阴性。这些结果共同表明，子宫基质细胞中的FGF信号转导是由FGFR1的激活介导的。
