黑腹果蝇63E - 64A基因组区域的遗传分析：复制因子C亚基中突变的鉴定
A genetic analysis of the 63E-64A genomic region of Drosophila melanogaster: identification of mutations in a replication factor C subunit.
作者信息
Harrison S D, Solomon N, Rubin G M
机构信息
Howard Hughes Medical Institute, University of California, Berkeley 94720-3200, USA.
出版信息
Genetics. 1995 Apr;139(4):1701-9. doi: 10.1093/genetics/139.4.1701.
Abstract
We have performed an F2 genetic screen to identify lethal mutations within the 63E-64A genomic region. We have isolated 122 mutations in 20 different complementation groups. Of these groups, 16 are represented by multiple alleles. We have also established that the Rop and Ras2 genes are located within the 63E-64A genomic domain at 64A10,11. We have sequenced 10.2 kb of DNA surrounding this gene pair and find that in addition to Rop and Ras2 there is another gene located within this DNA sequence. The gene product, which we have named Rfc40, shows 68% identity to the 40-kDa subunit of replication factor C. We find that the members of one complementation group (13 alleles) derived from our screen correspond to mutations in the Rop gene, whereas the members of another (five alleles) correspond to mutations in the Rfc40 gene. In addition we have isolated 11 new mutant alleles of the disembodied gene.
摘要
我们进行了一次F2遗传筛选，以鉴定63E - 64A基因组区域内的致死突变。我们在20个不同的互补群中分离出了122个突变。在这些互补群中，16个由多个等位基因代表。我们还确定Rop和Ras2基因位于64A10、11处的63E - 64A基因组区域内。我们对这一对基因周围10.2 kb的DNA进行了测序，发现除了Rop和Ras2之外，该DNA序列中还存在另一个基因。我们将该基因产物命名为Rfc40，它与复制因子C的40 kDa亚基有68%的同源性。我们发现，从我们的筛选中得到的一个互补群（13个等位基因）的成员对应于Rop基因中的突变，而另一个互补群（5个等位基因）的成员对应于Rfc40基因中的突变。此外，我们还分离出了11个新的“无形”基因的突变等位基因。
相似文献
1
A genetic analysis of the 63E-64A genomic region of Drosophila melanogaster: identification of mutations in a replication factor C subunit.黑腹果蝇63E - 64A基因组区域的遗传分析：复制因子C亚基中突变的鉴定
Genetics. 1995 Apr;139(4):1701-9. doi: 10.1093/genetics/139.4.1701.
2
Structure-specific abnormalities associated with mutations in a DNA replication accessory factor in Drosophila.
Dev Biol. 2001 Feb 15;230(2):161-76. doi: 10.1006/dbio.2000.0117.
3
The uvsF gene region in Aspergillus nidulans codes for a protein with homology to DNA replication factor C.构巢曲霉中的uvsF基因区域编码一种与DNA复制因子C具有同源性的蛋白质。
Gene. 1997 Jun 3;191(2):155-9. doi: 10.1016/s0378-1119(97)00052-8.
4
The Schizosaccharomyces pombe rfc3+ gene encodes a homologue of the human hRFC36 and Saccharomyces cerevisiae Rfc3 subunits of replication factor C.粟酒裂殖酵母rfc3+基因编码人hRFC36和酿酒酵母复制因子C的Rfc3亚基的同源物。
Curr Genet. 2000 Mar;37(3):159-67. doi: 10.1007/s002940050514.
5
6
Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans.小鼠和人类复制因子C 140千道尔顿亚基的克隆、表达及染色体定位
Mol Cell Biol. 1994 Mar;14(3):1626-34. doi: 10.1128/mcb.14.3.1626-1634.1994.
7
Characterization of the five replication factor C genes of Saccharomyces cerevisiae.酿酒酵母五个复制因子C基因的特性分析。
Mol Cell Biol. 1995 Sep;15(9):4661-71. doi: 10.1128/MCB.15.9.4661.
8
Studies of the cloned 37-kDa subunit of activator 1 (replication factor C) of HeLa cells.对HeLa细胞激活因子1（复制因子C）的37-kDa克隆亚基的研究。
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5211-5. doi: 10.1073/pnas.89.12.5211.
9
Cloning of the large subunit of activator 1 (replication factor C) reveals homology with bacterial DNA ligases.激活因子1（复制因子C）大亚基的克隆揭示了其与细菌DNA连接酶的同源性。
Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11543-7. doi: 10.1073/pnas.90.24.11543.
10
The RFC2 gene encoding a subunit of replication factor C of Saccharomyces cerevisiae.编码酿酒酵母复制因子C一个亚基的RFC2基因。
Nucleic Acids Res. 1994 May 11;22(9):1527-35. doi: 10.1093/nar/22.9.1527.
引用本文的文献
1
sustains trans-synaptic BMP signaling required for synaptic maintenance with age.维持跨突触 BMP 信号传导，这是突触维持所必需的，随着年龄的增长而减少。
Elife. 2021 Mar 5;10:e54932. doi: 10.7554/eLife.54932.
2
Differential regulation of dendritic and axonal development by the novel Krüppel-like factor Dar1.新型 Krüppel 样因子 Dar1 对树突和轴突发育的差异调控。
J Neurosci. 2011 Mar 2;31(9):3309-19. doi: 10.1523/JNEUROSCI.6307-10.2011.
3
The Cdc42-selective GAP rich regulates postsynaptic development and retrograde BMP transsynaptic signaling.Cdc42 选择性 GAP 丰富调节突触后发育和逆行 BMP 突触间信号转导。
J Cell Biol. 2010 Nov 1;191(3):661-75. doi: 10.1083/jcb.201007086.
4
Ero1L, a thiol oxidase, is required for Notch signaling through cysteine bridge formation of the Lin12-Notch repeats in Drosophila melanogaster.Ero1L是一种硫醇氧化酶，果蝇中通过Lin12-Notch重复序列的半胱氨酸桥形成来进行Notch信号传导需要Ero1L。
J Cell Biol. 2008 Sep 22;182(6):1113-25. doi: 10.1083/jcb.200805001.
5
DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex.DNA损伤诱导复制因子C复合物的RFC2亚基发生泛素化。
J Biol Chem. 2008 Apr 4;283(14):9071-9. doi: 10.1074/jbc.M709835200. Epub 2008 Feb 1.
6
Reduced growth of Drosophila neurofibromatosis 1 mutants reflects a non-cell-autonomous requirement for GTPase-Activating Protein activity in larval neurons.果蝇神经纤维瘤病1型突变体的生长减缓反映了幼虫神经元中对GTP酶激活蛋白活性的非细胞自主性需求。
Genes Dev. 2006 Dec 1;20(23):3311-23. doi: 10.1101/gad.1466806. Epub 2006 Nov 17.
7
ACK family tyrosine kinase activity is a component of Dcdc42 signaling during dorsal closure in Drosophila melanogaster.ACK家族酪氨酸激酶活性是黑腹果蝇背侧闭合过程中Dcdc42信号传导的一个组成部分。
Mol Cell Biol. 2002 Jun;22(11):3685-97. doi: 10.1128/MCB.22.11.3685-3697.2002.
8
Loss of cell cycle checkpoint control in Drosophila Rfc4 mutants.果蝇Rfc4突变体中细胞周期检查点控制的丧失。
Mol Cell Biol. 2001 Aug;21(15):5156-68. doi: 10.1128/MCB.21.15.5156-5168.2001.
9
Identification of chromosome inheritance modifiers in Drosophila melanogaster.黑腹果蝇中染色体遗传修饰因子的鉴定。
Genetics. 2001 Apr;157(4):1623-37. doi: 10.1093/genetics/157.4.1623.
10
Differential activity of Ras1 during patterning of the Drosophila dorsoventral axis.Ras1在果蝇背腹轴模式形成过程中的差异活性。
Genetics. 1996 Dec;144(4):1545-57. doi: 10.1093/genetics/144.4.1545.
本文引用的文献
1
The Drosophila Ras2 and Rop gene pair: a dual homology with a yeast Ras-like gene and a suppressor of its loss-of-function phenotype.果蝇Ras2和Rop基因对：与酵母类Ras基因及其功能缺失表型抑制子的双重同源性。
Development. 1993 Apr;117(4):1309-19. doi: 10.1242/dev.117.4.1309.
2
Analysis of genetic mosaics in developing and adult Drosophila tissues.发育中和成年果蝇组织中基因镶嵌体的分析。
Development. 1993 Apr;117(4):1223-37. doi: 10.1242/dev.117.4.1223.
3
Molecular cloning and expression of the Saccharomyces cerevisiae RFC3 gene, an essential component of replication factor C.酿酒酵母复制因子C的必需组分RFC3基因的分子克隆与表达
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):868-72. doi: 10.1073/pnas.91.3.868.
4
A new five-year plan for the U.S. Human Genome Project.美国人类基因组计划新的五年计划。
Science. 1993 Oct 1;262(5130):43-6. doi: 10.1126/science.8211127.
5
Drosophila GABAergic systems. II. Mutational analysis of chromosomal segment 64AB, a region containing the glutamic acid decarboxylase gene.果蝇γ-氨基丁酸能系统。II. 64AB染色体区段的突变分析，该区段包含谷氨酸脱羧酶基因。
Mol Gen Genet. 1994 Jun 3;243(5):555-64. doi: 10.1007/BF00284204.
6
Mutations in the Drosophila Rop gene suggest a function in general secretion and synaptic transmission.
Neuron. 1994 Sep;13(3):555-66. doi: 10.1016/0896-6273(94)90025-6.
7
Mosaic analysis using FLP recombinase.使用FLP重组酶的镶嵌分析。
Methods Cell Biol. 1994;44:655-81. doi: 10.1016/s0091-679x(08)60937-1.
8
The Drosophila ras oncogenes: structure and nucleotide sequence.果蝇ras癌基因：结构与核苷酸序列。
Cell. 1984 Jul;37(3):1027-33. doi: 10.1016/0092-8674(84)90437-9.
9
Analysis of P transposable element functions in Drosophila.果蝇中P转座因子功能的分析。
Cell. 1984 Aug;38(1):135-46. doi: 10.1016/0092-8674(84)90534-8.
10
Characterization and developmental expression of a Drosophila ras oncogene.果蝇ras癌基因的特征及发育表达
Mol Cell Biol. 1985 Apr;5(4):885-9. doi: 10.1128/mcb.5.4.885-889.1985.
Zotero 插件