百日咳博德特氏菌cpn10/cpn60（groESL）同源物的克隆与测序
Cloning and sequencing of the Bordetella pertussis cpn10/cpn60 (groESL) homolog.
作者信息
Fernandez R C, Weiss A A
机构信息
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, OH 45267, USA.
出版信息
Gene. 1995 May 26;158(1):151-2. doi: 10.1016/0378-1119(95)00106-g.
The nucleotide sequence downstream from the Bordetella resistance to killing (brk) locus in Bordetella pertussis was determined. Analysis of the sequence revealed an operon consisting of two highly predicted open reading frames (ORFs). The deduced amino-acid sequence of each ORF has strong homologies to the heat-shock proteins/chaperonins Cpn60 and Cpn10. The promoter contains consensus sequences for both sigma 32 and sigma 70 binding, and it possesses the CIRCE regulatory inverted repeat. A potential Rho-independent terminator was identified and appears to be shared with the brkA gene.
测定了百日咳博德特氏菌中博德特氏菌抗杀伤（brk）位点下游的核苷酸序列。对该序列的分析揭示了一个由两个高度预测的开放阅读框（ORF）组成的操纵子。每个ORF推导的氨基酸序列与热休克蛋白/伴侣蛋白Cpn60和Cpn10具有很强的同源性。启动子包含σ32和σ70结合的共有序列，并且具有CIRCE调控反向重复序列。鉴定出一个潜在的不依赖Rho的终止子，并且它似乎与brkA基因共享。
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