Roth M
Ric Clin Lab. 1975 Apr-Jun;5(2):97-104. doi: 10.1007/BF02910119.
The various peptidases secreted by such exocrine tissues as gastric mucosa, pancreas and prostate are usually determined by catalytic methods. Another approach utilizes immunoassay. Endopeptidases were formerly assayed with protein substrates such as hemoglobin and albumin. These techniques are increasingly replaced by more specific ones using artificial peptide derivatives as substrates, some of which allow an increase in absorbance or fluorescence to be continuously recorded. The presently available methods of assaying pepsin, pancreatic trypsin, trypsinogen and carboxypeptidase A, enterokinase and several peptidases of human sperm are reviewed.
由胃黏膜、胰腺和前列腺等外分泌组织分泌的各种肽酶通常通过催化方法来测定。另一种方法是利用免疫测定。内肽酶以前是用血红蛋白和白蛋白等蛋白质底物进行测定的。这些技术正越来越多地被使用人工肽衍生物作为底物的更特异的方法所取代，其中一些方法可以连续记录吸光度或荧光的增加。本文综述了目前可用的胃蛋白酶、胰蛋白酶、胰蛋白酶原、羧肽酶A、肠激酶以及人类精子的几种肽酶的测定方法。
