样本制备的改进提高了用于诊断软下疳的聚合酶链反应检测的灵敏度。
Alterations in sample preparation increase sensitivity of PCR assay for diagnosis of chancroid.
作者信息
Johnson S R, Martin D H, Cammarata C, Morse S A
机构信息
Division of Sexually Transmitted Disease Laboratory Research, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1995 Apr;33(4):1036-8. doi: 10.1128/jcm.33.4.1036-1038.1995.
Abstract
A PCR assay for the detection of Haemophilus ducreyi in clinical specimens taken from genital ulcers was developed. Although H. ducreyi, when present in such specimens, could be detected by PCR, the sensitivity of the assay was reduced by the presence of Taq polymerase inhibitors in the specimen. The sensitivity of the PCR assay was improved by the use of detergents in preparing nuclei acids from clinical specimens and by the inclusion of a dialysis step prior to amplification. In addition, sodium phosphate included in the transport medium was found to be an inhibitor of the Taq polymerase.
摘要
开发了一种用于检测从生殖器溃疡采集的临床标本中杜克雷嗜血杆菌的聚合酶链反应（PCR）检测方法。虽然当此类标本中存在杜克雷嗜血杆菌时可通过PCR检测到，但标本中Taq聚合酶抑制剂的存在会降低该检测方法的灵敏度。通过在从临床标本制备核酸时使用去污剂以及在扩增前加入透析步骤，提高了PCR检测方法的灵敏度。此外，发现运输培养基中所含的磷酸钠是Taq聚合酶的一种抑制剂。
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本文引用的文献
1
Development of the polymerase chain reaction for diagnosis of chancroid.用于诊断软下疳的聚合酶链反应的开发。
J Clin Microbiol. 1993 Mar;31(3):659-64. doi: 10.1128/jcm.31.3.659-664.1993.
2
Transport media for Haemophilus ducreyi.杜克雷嗜血杆菌的运送培养基。
Sex Transm Dis. 1993 Jan-Feb;20(1):5-9. doi: 10.1097/00007435-199301000-00002.
3
Development of a polymerase chain reaction assay for the detection of Haemophilus ducreyi.
Sex Transm Dis. 1994 Jan-Feb;21(1):13-23. doi: 10.1097/00007435-199401000-00004.
4
Isolation and identification of Haemophilus ducreyi in a clinical study.一项临床研究中杜克雷嗜血杆菌的分离与鉴定
J Clin Microbiol. 1980 Aug;12(2):170-4. doi: 10.1128/jcm.12.2.170-174.1980.
5
The polymerase chain reaction, a review of the practical limitations for human immunodeficiency virus diagnosis.
J Virol Methods. 1989 Aug;25(2):179-87. doi: 10.1016/0166-0934(89)90031-1.
6
Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system.利用聚合酶链反应和非放射性检测系统检测临床样本中的结核分枝杆菌。
J Clin Microbiol. 1992 Oct;30(10):2567-75. doi: 10.1128/jcm.30.10.2567-2575.1992.
7
Comparison of specimen collection and laboratory techniques for isolation of Haemophilus ducreyi.用于分离杜克雷嗜血杆菌的标本采集与实验室技术比较
J Clin Microbiol. 1978 Jan;7(1):39-43. doi: 10.1128/jcm.7.1.39-43.1978.
8
The microbiological flora of penile ulcerations.
J Infect Dis. 1978 Jan;137(1):50-6. doi: 10.1093/infdis/137.1.50.
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