Fischer B, Retchkiman I, Bauer J, Platt D, Popa-Wagner A
Institute of Gerontology, University of Erlangen-Nürnberg, Germany.
J Neurochem. 1995 Jul;65(1):467-70. doi: 10.1046/j.1471-4159.1995.65010467.x.
Stimuli that evoke seizure are capable of inducing structural changes in the hippocampus. However, late-acting genes related to these changes have not been described. Administration of pentylenetetrazole (PTZ; 50 mg/kg) to rats of various ages evoked tonic-clonic seizures. Using RNA gel blot analysis we found that the level of the mRNA for microtubule-associated protein 1B (MAP1B) was robustly increased in the hippocampus of 3-month-old rats. The levels of MAP1B mRNA in hippocampus peaked at 40 h and began to decline by 72 h following PTZ treatment. Immunoblotting with anti-MAP1B antibody demonstrates the increase in content of immunoreactive proteins 40-72 h after seizure onset in the hippocampus of PTZ-treated rats. These results indicate that MAP1B is a sensitive indicator of hippocampal structural changes occurring in response to PTZ-induced seizure activity.
诱发癫痫发作的刺激能够在海马体中诱导结构变化。然而，与这些变化相关的晚期作用基因尚未被描述。给不同年龄的大鼠注射戊四氮（PTZ；50mg/kg）可诱发强直阵挛性癫痫发作。通过RNA凝胶印迹分析，我们发现微管相关蛋白1B（MAP1B）的mRNA水平在3个月大的大鼠海马体中显著升高。PTZ治疗后，海马体中MAP1B mRNA水平在40小时达到峰值，并在72小时开始下降。用抗MAP1B抗体进行免疫印迹显示，在PTZ处理大鼠的海马体中，癫痫发作开始后40-72小时免疫反应性蛋白含量增加。这些结果表明，MAP1B是海马体对PTZ诱导的癫痫活动发生结构变化的敏感指标。
