Savard P, Tremblay M
Unité de Médecine Génétique et Moléculaire, Hospitalier de l'Université Laval, Québec, Canada.
J Mol Biol. 1995 Jun 23;249(5):879-89. doi: 10.1006/jmbi.1995.0345.
Morphogenesis and pattern formation are biological processes that rely on the expression of positional determinants to divide the embryo into compartments. Hox genes are among the selector genes that direct the mechanism of positional information. Here we report the molecular structure and pattern of expression of a new Hox C6 transcript in the adult newt. Molecular analysis showed that the gene transcribes a long primary transcript in both limb and tail regeneration territories and subsequently uses maturation events to produce two RNAs that share the same DNA binding domain and differ in their 5' extremity. Both RNAs were found in the limb and showed a proximal-distal gradient of expression. The tail sample showed accumulation of only one Hox C6 transcript. These results suggest that both transcriptional and post-transcriptional regulations are involved in the appendage-specific expression of Hox C6 in the adult newt. Finally, the adult frog, which has lost its regeneration capacity, also post-transcriptionally regulates the expression of Hox C6 in its appendages.
形态发生和模式形成是依赖位置决定因子的表达将胚胎划分为不同区域的生物学过程。Hox基因是指导位置信息机制的选择基因之一。在此，我们报告成年蝾螈中一种新的Hox C6转录本的分子结构和表达模式。分子分析表明，该基因在肢体和尾部再生区域均转录出一条长的初级转录本，随后通过成熟事件产生两种RNA，它们共享相同的DNA结合结构域，但5'末端不同。两种RNA均在肢体中被发现，并呈现出近端到远端的表达梯度。尾部样本仅显示一种Hox C6转录本的积累。这些结果表明，转录调控和转录后调控均参与成年蝾螈中Hox C6在附肢特异性表达。最后，已失去再生能力的成年青蛙在其附肢中也对Hox C6的表达进行转录后调控。
