通过在合成毛细管上进行连续细胞培养生产“快速收获”莫洛尼鼠白血病病毒。
Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries.
作者信息
Ratner P L, Cleary M L, James E
出版信息
J Virol. 1978 May;26(2):536-9. doi: 10.1128/JVI.26.2.536-539.1978.
Abstract
Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.
摘要
莫洛尼鼠白血病病毒在从培养于合成毛细血管束上的慢性感染的NIH/3T3细胞（克隆1）中释放后的60分钟内自动收获。通过逆转录酶活性测定和XC合胞体试验测定的病毒产量表明，从生长长达10天的NIH/3T3细胞（克隆1）中回收了高传染性的“快速收获”病毒。
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