人血清对组织培养中人类前列腺肿瘤细胞摄取³H-胸腺嘧啶核苷的影响。
The effect of human serum on 3H-thymidine incorporation in human prostate tumors in tissue culture.
作者信息
Boileau M, Keenan E, Kemp E, Lawson R, Hodges C V
出版信息
J Urol. 1978 Jun;119(6):777-9. doi: 10.1016/s0022-5347(17)57631-4.
Human prostatic tumors, 4 adenomas and 4 adenocarcinomas, were established in tissue culture. We used the incorporation of 3H-thymidine into deoxyribonucleic acid as a measure of cell activity to compare the effects of supplementing the culture media with human or fetal calf serum. 3H-thymidine incorporation was greater with human serum, with up to 120 per cent increase observed. The addition of dihydrotestosterone (10(-8)M.) did not enhance or inhibit 3H-thymidine incorporation.
在组织培养中建立了4例前列腺腺瘤和4例前列腺腺癌的人前列腺肿瘤模型。我们采用将³H-胸腺嘧啶核苷掺入脱氧核糖核酸的方法来衡量细胞活性，以比较用人血清或胎牛血清补充培养基的效果。用人血清时³H-胸腺嘧啶核苷的掺入量更高，观察到增加了多达120%。添加二氢睾酮（10⁻⁸M）并未增强或抑制³H-胸腺嘧啶核苷的掺入。
Zotero 插件