血管加压素和促肾上腺皮质激素释放激素在血管活性肠肽和胰高血糖素样肽诱导促肾上腺皮质激素和皮质酮分泌中的作用

Involvement of vasopressin and corticotropin-releasing hormone in VIP- and PHI-induced secretion of ACTH and corticosterone.

作者信息

Alexander L D, Sander L D

机构信息

Department of Physiology, Meharry Medical College, School of Graduate Studies and Research, Nashville, Tennessee 37208, USA.

出版信息

Neuropeptides. 1995 Mar;28(3):167-73. doi: 10.1016/0143-4179(95)90112-4.

DOI:10.1016/0143-4179(95)90112-4

PMID:7791960

Abstract

The relative roles of hypothalamic corticotropin-releasing-hormone (CRH) and vasopressin (AVP) as mediators of the stimulant effect of vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI) on ACTH and corticosterone (CORT) secretion, were examined using receptor blockade of endogenous CRH and AVP. ACTH and CORT secretion were stimulated 6- and 7-fold, respectively, by PVN infusion of VIP (3.0 nmol) and 6- and 9-fold, respectively, by PHI (3.0 nmol). ACTH and CORT stimulation by VIP were inhibited 78 and 72%, respectively, by pretreatment with the CRF antagonist, 59 and 57%, respectively, by pretreatment with the AVP antagonist and about 78% by combined pretreatment with the CRF and AVP antagonists. PHI-induced stimulation of ACTH and CORT was inhibited 89 and 81%, 73 and 59% and 93% by pretreatment with the CRF- or AVP-antagonist or combined administration, respectively. These results support the hypothesis that the activation of the hypothalamic-pituitary-adrenal (HPA) axis by VIP and PHI is mediated through the release of endogenous CRH. AVP also plays a role in this response, possibly by enhancing the activity of CRH in a synergistic manner.

摘要

采用内源性促肾上腺皮质激素释放激素（CRH）和血管升压素（AVP）的受体阻断法，研究了下丘脑促肾上腺皮质激素释放激素（CRH）和血管升压素（AVP）作为血管活性肠肽（VIP）和肽组氨酸异亮氨酸（PHI）对促肾上腺皮质激素（ACTH）和皮质酮（CORT）分泌刺激作用的介质的相对作用。通过向室旁核（PVN）注入VIP（3.0 nmol），ACTH和CORT的分泌分别增加了6倍和7倍；通过注入PHI（3.0 nmol），ACTH和CORT的分泌分别增加了6倍和9倍。VIP对ACTH和CORT的刺激作用分别被CRF拮抗剂预处理抑制了78%和72%，被AVP拮抗剂预处理分别抑制了59%和57%，而被CRF和AVP拮抗剂联合预处理抑制了约78%。PHI诱导的ACTH和CORT刺激作用分别被CRF拮抗剂、AVP拮抗剂预处理或联合给药抑制了89%和81%、73%和59%以及93%。这些结果支持了这样的假说，即VIP和PHI对下丘脑-垂体-肾上腺（HPA）轴的激活是通过内源性CRH的释放介导的。AVP在这一反应中也起作用，可能是以协同方式增强CRH的活性。