Savjak D, Pikula B, Muss W H, Hacker G W, Amidzic L, Hauser-Kronberger C, Lelic-Marinovic M, Dietze O
Institute of Pathological Anatomy, University Medical Center, Banjaluka, Bosna.
Pathol Res Pract. 1994 Dec;190(12):1162-8. doi: 10.1016/S0344-0338(11)80442-5.
The nuclear specific surface density (Sv/Vv), mean nuclear area (A) and numerical density (Nv) of tumor cell nuclei from 30 primary invasive female breast carcinomas with known concentrations of estrogen and progesterone receptors (ER and PR), were morphometrically analyzed at the ultrastructural level. It was found that carcinomas with concordant positive ER and PR status contained significantly higher number of smaller nuclei per volume unit of epithelium (mean Nv = 1.5 x 10(6) mm-3, mean A = 27 microns 2), than carcinomas of negative concordant receptor status (mean Nv = 0.8 x 10(6) mm-3, mean = 37 microns 2). Tumor cell nuclei of the former frequently displayed an elliptic shape (mean Sv/Vv = 1.16 microns-1), and had deeply invaginated surfaces, whereas nuclei of the latter were more frequently ball-shaped and exhibited a smooth-surface (mean Sv/Vv = 0.88 microns-1). The numerical density Nv (NUC/EPI) of tumor cell nuclei turned out to be a most reliable morphological marker of the concordant ER and PR status (overall efficiency = 84%, p < 0.01). The nuclear surface and volume ratio and mean nuclear area also appear to be reliable markers for terminal stages of the biochemical differentiation of breast carcinoma (overall efficiency = 79%, p < 0.05).
对30例已知雌激素和孕激素受体（ER和PR）浓度的原发性浸润性女性乳腺癌的肿瘤细胞核，在超微结构水平进行形态计量分析，测定其核比表面密度（Sv/Vv）、平均核面积（A）和核数密度（Nv）。结果发现，ER和PR状态一致为阳性的癌组织，每单位上皮体积内的小核数量显著多于受体状态一致为阴性的癌组织（平均Nv = 1.5×10⁶mm⁻³，平均A = 27μm²），后者的平均Nv = 0.8×10⁶mm⁻³，平均A = 37μm²。前者的肿瘤细胞核常呈椭圆形（平均Sv/Vv = 1.16μm⁻¹），表面深陷，而后者的细胞核多为球形，表面光滑（平均Sv/Vv = 0.88μm⁻¹）。肿瘤细胞核的数密度Nv（NUC/EPI）是ER和PR状态一致的最可靠形态学标志物（总效率 = 84%，p < 0.01）。核表面积与体积比以及平均核面积似乎也是乳腺癌生化分化终末期的可靠标志物（总效率 = 79%，p < 0.05）。
