[痢疾噬菌体的免疫化学和分子生物学特性]

[Immunochemical and molecular biological characteristics of dysentery phages].

作者信息

Zamchuk L A, Magradze N M

出版信息

Vopr Virusol. 1976 Mar-Apr(2):161-6.

Abstract

Molecular organization of DNA of six dysentery phages was studied for their classification. The pattern of glucosilation of their DNA was determined by an immunochemical method and the test of competitive hybridization established the degree of homology of these DNA in relation to T4 phage DNA. Four of the phages (DDVI, Zonne "Gorkyi" Zonne "Czechoslovakia", Zonne "Poland") were shown to belong to the group of T-even phages by their pattern of DNA glucosilation and by their capacity to inhibit DNA T4X3H DNA T4 hybridization. According to these criteria, two other phages (Zonne "Ufa" and Zonne "Khabarovsk") do not belong to the group of T-even phages.

摘要

为了对六种痢疾噬菌体进行分类，研究了它们DNA的分子结构。通过免疫化学方法确定了它们DNA的糖基化模式，竞争杂交试验确定了这些DNA与T4噬菌体DNA的同源程度。其中四种噬菌体（DDVI、高尔基区噬菌体、捷克斯洛伐克区噬菌体、波兰区噬菌体）通过其DNA糖基化模式以及抑制T4X3H DNA与T4 DNA杂交的能力，被证明属于T偶数噬菌体组。根据这些标准，另外两种噬菌体（乌法区噬菌体和哈巴罗夫斯克区噬菌体）不属于T偶数噬菌体组。