Rosenberg S M, Wollenzien T F, Johnson M M, Eberly L, Hurley C K, Reinsmoen N L, Steiner N, Goeken N E
Tissue Typing Lab, VAMC, Iowa City, USA.
Tissue Antigens. 1995 Feb;45(2):125-8. doi: 10.1111/j.1399-0039.1995.tb02429.x.
We have discovered a previously unpublished HLA-DRB1 allele, observed in a patient (SB), his mother, and one sibling. The undefined allele gave sporadic positive reactions with sera in the DR52-associated group. SSOPH analysis utilizing both generic and group specific primers and probes also gave ambiguous results. SB typed clearly as a DRB10301 (paternal allele) but the DNA from SB also bound probes specific for DRB114 and DRB111. Sequencing revealed that the undefined allele was similar to a DRB114 allele with a segment of sequence found in DRB111 alleles. The patient was MLC reactive with donors who express DRB10301, *1401 and *0301, 11 and was nonreactive solely to DRB10301 (Dw3) homozygous typing cells.
我们发现了一个此前未发表的HLA - DRB1等位基因，在一名患者（SB）、其母亲和一个兄弟姐妹中观察到。该未定义的等位基因与DR52相关组中的血清产生散发性阳性反应。使用通用引物和组特异性引物及探针的SSOPH分析也得出了不明确的结果。SB明确分型为DRB10301（父本等位基因），但来自SB的DNA也与DRB114和DRB111的特异性探针结合。测序显示，该未定义的等位基因与一个DRB114等位基因相似，其一段序列在DRB111等位基因中也有发现。该患者与表达DRB10301、1401以及0301、11的供体发生混合淋巴细胞反应，并且仅对DRB10301（Dw3）纯合分型细胞无反应。
