Piovesan P, Quatrini G, Pacifici L, Taglialatela G, Angelucci L
Institute for Research on Senescence, Sigma-Tau, Pomezia, Italy.
Int J Dev Neurosci. 1995 Feb;13(1):13-9. doi: 10.1016/0736-5748(94)00070-j.
Transection of the fimbria-fornix bundle in adult rats results in degeneration of the septohippocampal cholinergic pathway, reminiscent of that occurring in aging as well as Alzheimer disease. We report here a study of the effect of a treatment with acetyl-L-carnitine (ALCAR) in three-month-old Fischer 344 rats bearing a partial unilateral fimbria-fornix transection. ALCAR is known to ameliorate some morphological and functional disturbances in the aged central nervous system (CNS). We used choline acetyltransferase (ChAT) and acetyl cholinesterase (AChE) as markers of central cholinergic function, and nerve growth factor (NGF) levels as indicative of the trophic regulation of the medio-septal cholinergic system. ChAT and AChE activities were significantly reduced in the hippocampus (HIPP) ipsilateral to the lesion as compared to the contralateral one, while no changes were observed in the septum (SPT), nucleus basalis magnocellularis (NBM) or frontal cortex (FCX). ALCAR treatment restored ChAT activity in the ipsilateral HIPP, while AChE levels were not different from those of untreated animals, and did not affect NGF content in either SPT or HIPP.
成年大鼠穹窿-海马伞束横断会导致隔海马胆碱能通路退变，这与衰老以及阿尔茨海默病中发生的情况类似。我们在此报告一项关于在部分单侧穹窿-海马伞横断的三月龄Fischer 344大鼠中使用乙酰-L-肉碱（ALCAR）治疗效果的研究。已知ALCAR可改善老年中枢神经系统（CNS）中的一些形态和功能紊乱。我们使用胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）作为中枢胆碱能功能的标志物，并将神经生长因子（NGF）水平作为中隔胆碱能系统营养调节的指标。与对侧相比，损伤同侧海马（HIPP）中的ChAT和AChE活性显著降低，而在隔区（SPT）、基底大细胞核（NBM）或额叶皮质（FCX）中未观察到变化。ALCAR治疗恢复了同侧HIPP中的ChAT活性，而AChE水平与未治疗动物的水平无差异，并且不影响SPT或HIPP中的NGF含量。
