Merryweather-Clarke A T, Hirst M, Possee R D
NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
Acta Virol. 1994 Dec;38(6):311-5.
Trichoplusia ni larvae have been injected with a mixture of wild-type Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and a mutant derivative, AcRP8.UW1.lacZ, which lacks the polyhedrin gene, and has the p10 gene replaced by the Escherichia coli beta-galactosidase gene. Following plaque assay of the haemolymph and subsequent staining for beta-galactosidase activity and scoring for polyhedra, recombinant plaques were identified and the recombination frequency estimated as 6.6%.
已将野生型苜蓿银纹夜蛾多核多角体病毒（AcMNPV）和一种突变衍生物AcRP8.UW1.lacZ的混合物注射到粉纹夜蛾幼虫体内，该突变衍生物缺乏多角体蛋白基因，其p10基因被大肠杆菌β-半乳糖苷酶基因取代。在对血淋巴进行噬菌斑测定，随后对β-半乳糖苷酶活性进行染色并对多角体进行评分后，鉴定出重组噬菌斑，重组频率估计为6.6%。
