Takeuchi K, Yuta A, Sakakura Y
Department of Otorhinolaryngology, Mie University School of Medicine, Japan.
Am J Otolaryngol. 1995 Mar-Apr;16(2):98-102. doi: 10.1016/0196-0709(95)90039-x.
Interleukin-8 (IL-8), a monocyte-derived and macrophage-derived cytokine, displays potent chemotactic activating properties toward neutrophils and thus may contribute to the pathogenesis of chronic sinusitis. The object of this investigation was to show the expression of the IL-8 gene in chronic sinusitis by Northern blot analysis and a reverse transcription-polymerase chain reaction (RT-PCR).
For Northern blot analysis, RNAs were extracted from maxillary mucosa and nasal polyps from two patients with chronic sinusitis, respectively, and from the inferior turbinate of a nasal allergy patient. For RT-PCR, RNAs were extracted from 11 patients with chronic sinusitis, 8 patients with allergic rhinitis, and 4 patients with hypertrophic rhinitis.
Whereas IL-8 mRNA was expressed in the maxillary mucosa, IL-8 transcript was not detected in the inferior turbinate by Northern blot analysis. IL-8 transcripts were detected in 45% of chronic sinusitis RNAs (5/11) and in 50% of allergic rhinitis RNAs (5/10) by RT-PCR.
These data suggest IL-8 may contribute to neutrophil involvement in chronic sinusitis.
白细胞介素-8（IL-8）是一种由单核细胞和巨噬细胞产生的细胞因子，对中性粒细胞具有强大的趋化激活特性，因此可能在慢性鼻窦炎的发病机制中起作用。本研究的目的是通过Northern印迹分析和逆转录-聚合酶链反应（RT-PCR）来显示IL-8基因在慢性鼻窦炎中的表达。
用于Northern印迹分析的RNA分别从两名慢性鼻窦炎患者的上颌窦黏膜和鼻息肉以及一名鼻过敏患者的下鼻甲中提取。用于RT-PCR的RNA从11名慢性鼻窦炎患者、8名变应性鼻炎患者和4名肥厚性鼻炎患者中提取。
通过Northern印迹分析，虽然IL-8 mRNA在上颌窦黏膜中表达，但在下鼻甲中未检测到IL-8转录本。通过RT-PCR在45%的慢性鼻窦炎RNA（5/11）和50%的变应性鼻炎RNA（5/10）中检测到IL-8转录本。
这些数据表明IL-8可能在慢性鼻窦炎中导致中性粒细胞的参与。
