Usha A P, Simpson S P, Williams J L
Roslin Institute, Edinburgh Research Station, Midlothian, UK.
Anim Genet. 1995 Jun;26(3):155-61. doi: 10.1111/j.1365-2052.1995.tb03155.x.
Many microsatellite sequences have been described in the bovine genome. Being highly polymorphic these have been suggested as markers for parentage verification and individual identification in cattle. We have evaluated the use of five highly polymorphic microsatellite markers for parentage verification in 14 breeds of cattle in the UK. Three of the microsatellite loci occur within introns in genes: BoLA DRB3, steroid 21-hydroxylase, and the beta subunit of the follicle-stimulating hormone. The other two are anonymous sites ETH131 and HEL6. Results were analysed by a statistical approach that takes in to account deviations from Hardy-Wienberg equilibrium and linkage disequilibrium for multiple loci. The method of determining the probability of random sire exclusion uses observed genotype frequencies instead of allele frequencies. Independently, the markers used have a probability of between 0.72 and 0.62 of identifying a parentage error, while used together the five markers give, on average across breeds, a probability of 0.99 of excluding an incorrect sire.
在牛基因组中已描述了许多微卫星序列。由于这些序列具有高度多态性，因此被建议作为牛亲子鉴定和个体识别的标记。我们评估了5个高度多态性微卫星标记在英国14个牛品种亲子鉴定中的应用。其中3个微卫星位点位于基因的内含子中：牛白细胞抗原DRB3、类固醇21-羟化酶和促卵泡激素的β亚基。另外两个是无名位点ETH131和HEL6。通过一种统计方法对结果进行分析，该方法考虑了多个位点偏离哈迪-温伯格平衡和连锁不平衡的情况。确定随机排除父系概率的方法使用观察到的基因型频率而非等位基因频率。单独使用时，所使用的标记识别亲子关系错误的概率在0.72至0.62之间，而一起使用时，这5个标记在各品种中的平均排除错误父系概率为0.99。
