大鼠腮腺中环状AMP磷酸二酯酶同工酶的特性研究
Characterization of cyclic AMP phosphodiesterase isozymes in rat parotid gland.
作者信息
Imai A, Nashida T, Shimomura H
机构信息
Department of Oral Biochemistry, Nippon Dental University, School of Dentistry at Niigata, Japan.
出版信息
Arch Oral Biol. 1995 Feb;40(2):165-8. doi: 10.1016/0003-9969(94)00139-3.
PMID:7794130
Abstract
Cyclic AMP phosphodiesterase (PDE) isozymes were isolated and characterized from the soluble fraction of rat parotid gland. Four main peaks containing PDE activity were obtained by Q-Sepharose Fast Flow column chromatography. The four peaks were identified as PDEs I-IV by kinetic properties, molecular-weight analysis and their responses to effectors and inhibitors.
摘要
环磷酸腺苷磷酸二酯酶（PDE）同工酶从大鼠腮腺的可溶性部分中分离并鉴定。通过Q-Sepharose Fast Flow柱色谱法获得了四个含有PDE活性的主峰。根据动力学性质、分子量分析及其对效应物和抑制剂的反应，将这四个峰鉴定为PDE I-IV。
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