Holt P S, Macri N P, Porter R E
U.S. Department of Agriculture, Southeast Poultry Research Laboratory, Athens, Georgia 30605, USA.
Avian Dis. 1995 Jan-Mar;39(1):55-63.
A study was conducted in which the early kinetics (4 hr to 96 hr) of an infection by Salmonella enteritidis in older white leghorn hens was examined, and a molt was induced through withholding feed to determine its effect on the progression of this infection. Molted and unmolted hens were orally infected with 5-10 x 10(6) S. enteritidis on day 4 of the feed removal. At 4, 24, 48, 72, and 96 hr postinfection, liver, spleen, ileum, colon, cecum, and feces were removed from six hens per group and sampled for the presence of the challenge organism. By 24 hr postinfection, S. enteritidis was most prevalent in the cecum and feces of unmolted hens, and this prevalence continued throughout the experimental period. In molted hens, however, S. enteritidis could be detected in a high percentage (90-100%) of colon, cecum, and feces samples at 24 to 96 hr postinfection and in 67% or more of ileum samples at 48 to 96 hr postinfection, indicating a much wider distribution of the S. enteritidis along the intestinal tract than in unmolted hens. The numbers of S. enteritidis recovered from these alimentary samples were also significantly higher in molted than unmolted hens. S. enteritidis could not be detected in livers or spleens of either treatment group at 4 or 24 hr postinfection. At 48, 72, and 96 hr postinfection, 50% or more of the livers and spleens in both the molted and unmolted hens were positive for the challenge organism, but significantly more S. enteritidis was recovered from the organs of the molted hens at these three sampling times.(ABSTRACT TRUNCATED AT 250 WORDS)
进行了一项研究，检测了老年白来航鸡感染肠炎沙门氏菌的早期动力学（4小时至96小时），并通过停食诱导换羽，以确定其对该感染进程的影响。在停食第4天，对换羽和未换羽的母鸡经口接种5 - 10×10⁶肠炎沙门氏菌。感染后4、24、48、72和96小时，从每组6只母鸡中取出肝脏、脾脏、回肠、结肠、盲肠和粪便，检测其中是否存在攻击菌。感染后24小时，肠炎沙门氏菌在未换羽母鸡的盲肠和粪便中最为普遍，且这种普遍性在整个实验期持续存在。然而，在换羽母鸡中，感染后24至96小时，90% - 100%的结肠、盲肠和粪便样本中可检测到肠炎沙门氏菌，感染后48至96小时，67%或更多的回肠样本中可检测到，这表明肠炎沙门氏菌在肠道中的分布比未换羽母鸡更广。从这些消化道样本中回收的肠炎沙门氏菌数量在换羽母鸡中也显著高于未换羽母鸡。感染后4或24小时，两个处理组的肝脏或脾脏中均未检测到肠炎沙门氏菌。感染后48、72和96小时，换羽和未换羽母鸡中50%或更多的肝脏和脾脏对攻击菌呈阳性，但在这三个采样时间，从换羽母鸡器官中回收的肠炎沙门氏菌明显更多。（摘要截断于250字）
