P2Y嘌呤受体刺激对大鼠肾脏糖异生的影响。

Effect of P2Y-purinoceptor stimulation on renal gluconeogenesis in rats.

作者信息

Cha S H, Jung K Y, Endou H

机构信息

Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1995 Jun 15;211(2):454-61. doi: 10.1006/bbrc.1995.1835.

DOI:10.1006/bbrc.1995.1835

PMID:7794257

Abstract

In the freshly prepared rat renal cortical tubule suspension, the effects of ATP on intracellular free calcium mobilization and gluconeogenesis were investigated. ATP increased intracellular free calcium concentration ([Ca2+]i) in a dose-dependent manner (10(-6) - 10(-3) M). The rank order in the potency of ATP analogs at 10(-4) M was 2-methylthio ATP > ATP > or = ADP. AMP, adenosine and alpha, beta-methylene ATP did not respond to [Ca2+]i. These results suggest that P2-purinoceptor in the rat kidney cortex should be specifically P2Y subtype. Renal gluconeogenesis from pyruvate was stimulated maximally by 10(-4) M ATP (48.3 +/- 13.2%). This effect was significantly inhibited by 10(-4) M suramin. Thus, the present study suggests that renal gluconeogenesis is increased via P2Y-specific purinoceptor stimulation.

摘要

在新鲜制备的大鼠肾皮质肾小管悬浮液中，研究了ATP对细胞内游离钙动员和糖异生的影响。ATP以剂量依赖方式（10(-6)-10(-3)M）增加细胞内游离钙浓度（[Ca2+]i）。10(-4)M时ATP类似物的效力排序为2-甲硫基ATP>ATP>或=ADP。AMP、腺苷和α,β-亚甲基ATP对[Ca2+]i无反应。这些结果表明，大鼠肾皮质中的P2嘌呤受体应为特异性P2Y亚型。丙酮酸的肾糖异生在10(-4)M ATP作用下受到最大刺激（48.3±13.2%）。该作用被10(-4)M苏拉明显著抑制。因此，本研究表明，通过P2Y特异性嘌呤受体刺激可增加肾糖异生。