Goasduff T, Menez J F, Dreano Y, Berthou F
Laboratoire de Biochimie-Nutrition, Equipe d'accueil DGRT EAD 948, Faculté de Médecine, Brest, France.
Biochem Biophys Res Commun. 1995 Jun 15;211(2):497-503. doi: 10.1006/bbrc.1995.1841.
The effects of combined ethanol and 3-methylcholanthrene treatment on rat hepatic cytochrome CYP1A2 and CYP2E1 expression were evaluated. Such a treatment attempts to mimic the simultaneous consumption of ethanol and cigarette smoke. Treatments involving 3-methylcholanthrene and combined ethanol + 3-methylcholanthrene decreased both CYP2E1 expression at the mRNA level (0.6 and 0.4 fold versus controls, respectively) and protein level (0.6 and 0.9 fold versus controls, respectively), while dramatically increasing CYP1A2 expression. Furthermore, combined treatment provokes a synergistic induction of CYP1A2 expression as determined by its catalytic activity and protein content.
评估了乙醇与3-甲基胆蒽联合处理对大鼠肝脏细胞色素CYP1A2和CYP2E1表达的影响。这种处理旨在模拟乙醇与香烟烟雾同时摄入的情况。涉及3-甲基胆蒽以及乙醇+3-甲基胆蒽联合处理均降低了CYP2E1在mRNA水平（分别为对照组的0.6倍和0.4倍）和蛋白质水平（分别为对照组的0.6倍和0.9倍）的表达，同时显著增加了CYP1A2的表达。此外，联合处理通过其催化活性和蛋白质含量测定，引发了CYP1A2表达的协同诱导。
