Tsiboli P, Choli T
Aristotle University of Thessaloniki, School of Chemistry, Laboratory of Biochemistry, Greece.
Biol Chem Hoppe Seyler. 1995 Feb;376(2):127-30. doi: 10.1515/bchm3.1995.376.2.127.
The amino acid sequence of the ribosomal protein S14 of Thermus thermophilus has been determined both by automated sequence analysis of the intact protein as well as by DNA sequence analysis of the gene. The carboxy-terminal region was verified by both amino acid sequence analysis of the carboxy-terminal peptide produced after Glu-C digestion and by DNA sequence analysis. The protein contains 60 amino acid residues with a calculated molecular weight of 7008. The most extensive homology is observed in the carboxy-terminal regions of all S14 proteins compared. Interestingly, the carboxy-terminal region of most S14 proteins of all species studied so far, form zinc-finger domains in the variety of C2-C2 form.
嗜热栖热菌核糖体蛋白S14的氨基酸序列已通过完整蛋白质的自动序列分析以及该基因的DNA序列分析得以确定。通过对Glu-C消化后产生的羧基末端肽进行氨基酸序列分析以及DNA序列分析，对羧基末端区域进行了验证。该蛋白质含有60个氨基酸残基，计算分子量为7008。在所比较的所有S14蛋白的羧基末端区域观察到最广泛的同源性。有趣的是，到目前为止所研究的所有物种的大多数S14蛋白的羧基末端区域，形成了多种C2-C2形式的锌指结构域。
